Conditional knock-out of β-catenin in postnatal-born dentate gyrus granule neurons results in dendritic malformation

被引:79
作者
Gao, Xiang [1 ]
Arlotta, Paola [3 ,4 ,5 ,6 ]
Macklis, Jeffrey D. [3 ,4 ,5 ,6 ]
Chen, Jinhui [1 ,2 ]
机构
[1] Univ Kentucky, Spinal Cord & Brain Injury Res Ctr, Lexington, KY 40536 USA
[2] Univ Kentucky, Dept Anat & Neurobiol, Lexington, KY 40536 USA
[3] Harvard Univ, Sch Med, Dept Neurosurg, Massachusetts Gen Hosp, Boston, MA 02114 USA
[4] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Neurol, Boston, MA 02114 USA
[5] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Neurosci Program, Boston, MA 02114 USA
[6] Harvard Univ, Sch Med, Ctr Nervous Syst Repair, Boston, MA 02114 USA
关键词
conditional knock-out; beta-catenin; dendritic development; newborns; dentate gyrus granule neurons; hippocampus;
D O I
10.1523/JNEUROSCI.3206-07.2007
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Neurons are continuously added to the brain throughout life, and these neurons must develop dendritic arbors and functional connections with existing neurons to be integrated into neuronal circuitry. The molecular mechanisms that regulate dendritic development of newborn neurons in the hippocampal dentate gyrus are still unclear. Here, we show that beta-catenin is expressed in newborn granule neurons and in neural progenitor cells in the hippocampal dentate gyrus. Specific knock-out of beta-catenin in newborn neurons, without affecting beta-catenin expression in neural progenitor cells, led to defects in dendritic morphology of these newborn neurons in vivo. Majority of newborn neurons that cannot extend dendrites survive < 1 month after they were born. Our results indicate that beta-catenin plays an important role in dendritic development of postnatal-born neurons in vivo, and is therefore essential for the neurogenesis in the postnatal brain.
引用
收藏
页码:14317 / 14325
页数:9
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