Impedimetric aptasensor for thrombin detection

被引：5

作者：

Meini, N. ^{[1
,2
]}

Farre, C. ^{[1
]}

Chaix, C. ^{[1
]}

Kherrat, K. ^{[2
]}

Dzyadevych, S. ^{[3
]}

Jaffrezic-Renault, N. ^{[1
]}

机构：

[1] Univ Lyon 1, Analyt Chem Lab, 43 Blvd 11 Novembre 1918, F-69622 Villeurbanne, France

[2] Badji Mokhtar Univ Annaba, Lab Wnvironm Engn, Annaba 23000, Algeria

[3] Natl Acad Sci Ukraine, Inst Mol Biol & Genet, Lab Biomol Elect, UA-03143 Kiev, Ukraine

来源：

EUROSENSORS XXV | 2011年 / 25卷

关键词：

aptamer; thrombin; electrochemical impedance spectroscopy (EIS); aptasensor;

D O I：

10.1016/j.proeng.2011.12.361

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The goal of this work is to develop an impedimetric aptasensor sensitive, selective and stable for detection of thrombin. Thiol modified aptamer sequence being grafted on gold electrode, the faradic electrochemical impedance measurements allow the direct detection of thrombin in the range of 3.1-200 ngmL(-1) with a detection limit of 3.1 ngmL(-1) (80 fM). This study demonstrates that these aptasensors exhibit high sensitivity, high selectivity (compared to a model protein, BSA), stability and reproducibility. (C) 2011 Published by Elsevier Ltd.

引用

页数：4