Enhancing bottom-up and top-down proteomic measurements with ion mobility separations

被引：55

作者：

Baker, Erin Shammel ^{[1
]}

Burnum-Johnson, Kristin E. ^{[1
]}

Ibrahim, Yehia M. ^{[1
]}

Orton, Daniel J. ^{[1
]}

Monroe, Matthew E. ^{[1
]}

Kelly, Ryan T. ^{[2
]}

Moore, Ronald J. ^{[1
]}

Zhang, Xing ^{[1
]}

Theberge, Roger ^{[3
]}

Costello, Catherine E. ^{[3
]}

Smith, Richard D. ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA

[2] Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA

[3] Boston Univ, Sch Med, Ctr Biomed Mass Spectrometry, Boston, MA 02118 USA

来源：

PROTEOMICS | 2015年 / 15卷 / 16期

关键词：

Ion mobility separations; Ion mobility spectrometry; Mass spectrometry; Proteomics; FLIGHT MASS-SPECTROMETRY; GAS-PHASE; BIOMARKER DISCOVERY; PROTEIN BIOMARKERS; TIME; THROUGHPUT; PLASMA; TRANSTHYRETIN; COMPLEXES; PIPELINE;

D O I：

10.1002/pmic.201500048

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteomic measurements with greater throughput, sensitivity, and structural information are essential for improving both in-depth characterization of complex mixtures and targeted studies. While LC separation coupled with MS (LC-MS) measurements have provided information on thousands of proteins in different sample types, the introduction of a separation stage that provides further component resolution and rapid structural information has many benefits in proteomic analyses. Technical advances in ion transmission and data acquisition have made ion mobility separations an opportune technology to be easily and effectively incorporated into LC-MS proteomic measurements for enhancing their information content. Herein, we report on applications illustrating increased sensitivity, throughput, and structural information by utilizing IMS-MS and LC-IMS-MS measurements for both bottom-up and top-down proteomics measurements.

引用

页码：2766 / 2776

页数：11

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