A novel 3D printing PCL/GelMA scaffold containing USPIO for MRI-guided bile duct repair

被引:41
作者
Li, Hehong [1 ]
Yin, Yingxian [2 ]
Xiang, Yang [3 ]
Liu, Haixia [4 ]
Guo, Rui [5 ]
机构
[1] Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Dept Radiol, Guangzhou 510623, Peoples R China
[2] Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Guangzhou Inst Pediat, Guangzhou 510623, Peoples R China
[3] Cent South Univ, Affiliated Haikou Hosp, Xiangya Med Coll, Dept Hepatobiliary Surg, Haikou 570100, Hainan, Peoples R China
[4] Guangzhou Nansha Dist Maternal & Child Hlth Care, Guangzhou 511458, Peoples R China
[5] Jinan Univ, Guangdong Prov Engn & Technol Res Ctr Drug Carrie, Key Lab Biomat Guangdong Higher Educ Inst, Dept Biomed Engn, Guangzhou 510632, Peoples R China
关键词
3D printing; PCL; gelatin methacryloyl (GelMA); bile duct; magnetic resonance imaging (MRI); TISSUE; DEGRADATION; CARTILAGE; HYDROGEL; DELIVERY; CELLS;
D O I
10.1088/1748-605X/ab797a
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Making artificial bile ducts in vitro for repairing and replacing diseased bile ducts is an important concept in tissue engineering. This study printed a tubular composite scaffold using polycaprolactone (PCL) through the current 3D printing method. It served as a matrix for the organoid cells of the bile duct to proliferation, migration, and differentiation. The PCL scaffold full of bile duct-like organ cells can achieve the effect of bionics, replacing the original bile duct to perform its proper function. In order to enrich the performance of the tubular scaffold, hydrogels were also used in this study. Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct. In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI). In this study, a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property was constructed by 3D printing. After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%. At the same time, the compression modulus of the stent could reach 17.41 kPa and the Young's modulus could reach 5.03 kPa. Thus, the mechanical properties of it can meet the needs of human implantation. USPIO can achieve MRI imaging in situ and nondestructively monitor the degradation of the stent in the body. In summary, PCL/GelMA/USPIO bile duct scaffolds are beneficial to the proliferation of cells on the scaffolds and can be used to construct biologically active artificial bile ducts.
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页数:11
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