Neurotransmitter Influence on Human Meibomian Gland Epithelial Cells

被引:55
作者
Kam, Wendy R. [1 ]
Sullivan, David A. [1 ]
机构
[1] Harvard Univ, Sch Med, Schepens Eye Res Inst, Boston, MA 02114 USA
关键词
IMMUNOREACTIVE NERVE-FIBERS; DRY EYE; INTERNATIONAL WORKSHOP; DYSFUNCTION REPORT; LACRIMAL GLAND; OCULAR SURFACE; MOUSE MODEL; ADENYLATE-CYCLASE; RAT CONJUNCTIVA; ACINAR-CELLS;
D O I
10.1167/iovs.11-8113
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
PURPOSE. A striking characteristic of the human meibomian gland is its rich sensory, sympathetic, and parasympathetic innervation, yet the functional relevance of these nerve fibers remains unknown. Acting on the hypothesis that neurotransmitters are released in the vicinity of the gland, act on glandular receptors, and influence the production, secretion, and/or delivery of meibomian gland secretions to the ocular surface, the goal in this study was to begin to determine whether neurotransmitters influence the meibomian gland. METHODS. Immortalized human meibomian gland epithelial (SLHMG) cells were examined for the presence of vasoactive intestinal peptide (VIP) and muscarinic acetylcholine (mACh) receptor transcripts and proteins. Cells were also exposed to VIP, carbachol, forskolin, and/or 3-isobutyl-1-methylxanthine (IBMX) to determine whether these agents, alone or in combination, modulate the adenylyl cyclase pathway, the accumulation of intracellular free calcium ([Ca(2+)](i)), or cell proliferation. RESULTS. Results demonstrate that SLHMG cells transcribe and translate VIP and mACh receptors; VIP, with either IBMX or forskolin, activates the adenylyl cyclase pathway, and the effect of VIP and forskolin together is synergistic; both VIP and carbachol increase intracellular [Ca(2+)] in SLHMG cells; and VIP with forskolin stimulates SLHMG cell proliferation. CONCLUSIONS. This study shows that parasympathetic neurotransmitters and their agonists influence the function of human meibomian gland epithelial cells. It remains to be determined whether this action alters the production, secretion, and/or delivery of meibum to the ocular surface. (Invest Ophthalmol Vis Sci. 2011;52:8543-8548) DOI: 10.1167/iovs.11-8113
引用
收藏
页码:8543 / 8548
页数:6
相关论文
共 59 条
[1]  
Aisa J, 2001, HISTOL HISTOPATHOL, V16, P487, DOI 10.14670/HH-16.487
[2]  
Andersson K-E, 2011, Handb Exp Pharmacol, P319, DOI 10.1007/978-3-642-16499-6_16
[3]  
[Anonymous], 2007, OCUL SURF, V5, P65
[4]   Detection of muscarinic receptor subtypes in human urinary bladder mucosa: Age and gender-dependent modifications [J].
Arrighi, Nicola ;
Bodei, Serena ;
Peroni, Angelo ;
Mirabella, Giuseppe ;
Zani, Danilo ;
Simeone, Claudio ;
Cunico, Sergio Cosciani ;
Spano, PierFranco ;
Sigala, Sandra .
NEUROUROLOGY AND URODYNAMICS, 2008, 27 (05) :421-428
[5]   FORSKOLIN STIMULATES ADENYLATE-CYCLASE IN HUMAN COLONIC CRYPTS - INTERACTION WITH VIP [J].
BOIGE, N ;
AMIRANOFF, B ;
MUNCK, A ;
LABURTHE, M .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1984, 101 (1-2) :111-117
[6]   Functional aspects of the tear film lipid layer [J].
Bron, AJ ;
Tiffany, JM ;
Gouveia, SM ;
Yokoi, N ;
Voon, LW .
EXPERIMENTAL EYE RESEARCH, 2004, 78 (03) :347-360
[7]   Chemical coding of sympathetic neurons controlling the tarsal muscle of the rat [J].
Chanthaphavong, RS ;
Murphy, SM ;
Anderson, CR .
AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL, 2003, 105 (02) :77-89
[8]  
Chung CW, 1996, INVEST OPHTH VIS SCI, V37, P238
[9]   Importance of the lipid layer in human tear film stability and evaporation [J].
Craig, JP ;
Tomlinson, A .
OPTOMETRY AND VISION SCIENCE, 1997, 74 (01) :8-13
[10]   VPAC and PAC receptors: From ligands to function [J].
Dickson, Louise ;
Finlayson, Keith .
PHARMACOLOGY & THERAPEUTICS, 2009, 121 (03) :294-316