3D-printable chitosan/silk fibroin/cellulose nanoparticle scaffolds for bone regeneration via M2 macrophage polarization

被引：85

作者：

Patel, Dinesh K. ^{[1
]}

Dutta, Sayan Deb ^{[2
]}

Hexiu, Jin ^{[3
]}

Ganguly, Keya ^{[2
]}

Lim, Ki-Taek ^{[1
,2
]}

机构：

[1] Kangwon Natl Univ, Inst Forest Sci, Chunchon 24341, South Korea

[2] Kangwon Natl Univ, Dept Biosyst Engn, Chunchon 24341, South Korea

[3] Capital Med Univ, Sch Stomatol, Beijing 100050, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2022年 / 281卷

基金：

新加坡国家研究基金会;

关键词：

3D-printed; Chitosan; Cellulose nanoparticles; Osteogenesis; Macrophages; Bone regeneration; SILK FIBROIN; OSTEOGENIC DIFFERENTIATION; NANOCOMPOSITE SCAFFOLDS; INFLAMMATORY RESPONSE; MECHANICAL-PROPERTIES; IN-VITRO; TISSUE; NANOFIBERS; NANOCRYSTALS; MODULATION;

D O I：

10.1016/j.carbpol.2021.119077

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Biopolymers-induced immune microenvironment exhibited prominent effects on bone regeneration. Osteoimmunomodulatory responses of cellulose nanoparticles incorporated chitosan hydrogel scaffolds have not yet been reported. The objective of this study was to monitor the synergistic effects of silk fibroin and cellulose nanoparticles on the immune-modulatory behavior of chitosan biopolymer scaffolds. 3D-printed biodegradable cellulose nanoparticles-reinforced chitosan/silk fibroin (CS/SF/CNPs) scaffolds were fabricated and characterized by different spectroscopic techniques. The improved rheological and recovery strength was observed in CS/ SF/CNPs hydrogels than pure polymer hydrogels. A significant shift from M1 -> M2 macrophages polarization occurred in the CS/SF/CNPs scaffolds treated groups than the control after 3 days of incubation, showing its immune-modulatory potential. Osteo-immunomodulatory effects of the fabricated scaffolds were analyzed on human bone marrow-derived mesenchymal stem cells (hBMSCs), with macrophages-derived conditioned media (M-CM). Enhanced bone regeneration was observed in the calvaria defect rat model, indicating that the fabricated scaffolds are promising materials for bone-healing applications.

引用

页数：18

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