4Pi-confocal microscopy of live cells

被引：52

作者：

Bahlmann, K ^{[1
]}

Jakobs, S ^{[1
]}

Hell, SW ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, High Resolut Opt Microscopy Grp, D-37070 Gottingen, Germany

来源：

ULTRAMICROSCOPY | 2001年 / 87卷 / 03期

关键词：

resolution; 4Pi; confocal; E; coli;

D O I：

10.1016/S0304-3991(00)00092-9

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

By coherently adding the spherical wavefronts of two opposing lenses, two-photon excitation 4Pi-confocal fluorescence microscopy has achieved three-dimensional imaging with an axial resolution 3-7 times better than confocal microscopy. So far this improvement was possible only in glycerol-mounted, fixed cells. Here we report 4Pi-confocal microscopy of watery objects and its application to the imaging of live cells. Water immersion of 4Pi-confocal microscopy of membrane stained live Escherichia coli bacteria attains a 4.3-fold better axial resolution as compared to the best water immersion confocal microscope. The resolution enhancement results into a vastly improved three-dimensional representation of the bacteria. The first images of live biological samples with an all-directional resolution in the 190-280 nm range are presented here, thus establishing a new resolution benchmark in live-cell microscopy. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：155 / 164

页数：10

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