Characterization of spermidine and spermine synthases in Lotus japonicus:: induction and spatial organization of polyamine biosynthesis in nitrogen fixing nodules

被引:23
作者
Efrose, R. C. [1 ,2 ]
Flemetakis, E. [1 ]
Sfichi, L. [3 ]
Stedel, C. [1 ]
Kouri, E. D. [1 ]
Udvardi, M. K. [4 ]
Kotzabasis, K. [3 ]
Katinakis, P. [1 ]
机构
[1] Agr Univ Athens, Dept Agr Biotechnol, Athens 11855, Greece
[2] Natl Ctr Sci Res Demokritos, Athens, Greece
[3] Univ Crete, Dept Biol, Iraklion 71409, Crete, Greece
[4] Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73402 USA
关键词
Lotus; polyamines; symbiotic nitrogen fixation; spermidine synthase; spermine synthase;
D O I
10.1007/s00425-008-0717-1
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The biosynthesis of the polyamines spermidine (Spd) and spermine (Spm) from putrescine (Put) is catalysed by the consequent action of two aminopropyltransferases, spermidine synthase (SPDS EC: 2.5.1.16) and spermine synthase (SPMS EC: 2.5.1.22). Two cDNA clones coding for SPDS and SPMS homologues in the nitrogen-fixing nodules of the model legume Lotus japonicus were identified. Functionality of the encoded polypeptides was confirmed by their ability to complement spermidine and spermine deficiencies in yeast. The temporal and spatial expression pattern of the respective genes was correlated with the accumulation of total polyamines in symbiotic and non-symbiotic organs. Expression of both genes was maximal at early stages of nodule development, while at later stages the levels of both transcripts declined. Both genes were expressed in nodule inner cortical cells, vascular bundles, and central tissue. In contrast to gene expression, increasing amounts of Put, Spd, and Spm were found to accumulate during nodule development and after maturity. Interestingly, nodulated plants exhibited systemic changes in both LjSPDS and LjSPMS transcript levels and polyamine content in roots, stem and leaves, in comparison to uninoculated plants. These results give new insights into the neglected role of polyamines during nodule development and symbiotic nitrogen fixation (SNF).
引用
收藏
页码:37 / 49
页数:13
相关论文
共 64 条
[1]   Differential expression of two spermidine synthase genes during early fruit development and in vegetative tissues of pea [J].
Alabadí, D ;
Carbonell, J .
PLANT MOLECULAR BIOLOGY, 1999, 39 (05) :933-943
[2]  
Alabadi D., 1999, Plant Physiology, V120, P935
[3]   Stress-induced changes in polyamine and tyramine levels can regulate proline accumulation in tomato leaf discs treated with sodium chloride [J].
Aziz, A ;
Martin-Tanguy, J ;
Larher, F .
PHYSIOLOGIA PLANTARUM, 1998, 104 (02) :195-202
[4]   Biosynthesis, oxidation and conjugation of aliphatic polyamines in higher plants [J].
Bagni, N ;
Tassoni, A .
AMINO ACIDS, 2001, 20 (03) :301-317
[5]  
BASTOLA DR, 1995, PLANT PHYSIOL, V109, P63, DOI 10.1104/pp.109.1.63
[6]  
Borrell A, 1996, PHYSIOL PLANTARUM, V98, P105, DOI 10.1111/j.1399-3054.1996.tb00680.x
[7]   Simultaneous reduction of the activity of two related enzymes, involved in early steps of the polyamine biosynthetic pathway, by a single antisense cDNA in transgenic rice [J].
Capell, T ;
Bassie, L ;
Topsom, L ;
Hitchin, E ;
Christou, P .
MOLECULAR AND GENERAL GENETICS, 2000, 264 (04) :470-476
[8]   Modulation of the polyamine biosynthetic pathway in transgenic rice confers tolerance to drought stress [J].
Capell, T ;
Bassie, L ;
Christou, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (26) :9909-9914
[9]   Over-expression of the oat arginine decarboxylase cDNA in transgenic rice (Oryza sativa L.) affects normal development patterns in vitro and results in putrescine accumulation in transgenic plants [J].
Capell, T ;
Escobar, C ;
Liu, H ;
Burtin, D ;
Lepri, O ;
Christou, P .
THEORETICAL AND APPLIED GENETICS, 1998, 97 (1-2) :246-254
[10]   Characterization and translational regulation of the arginine decarboxylase gene in carnation (Dianthus caryophyllus L.) [J].
Chang, KS ;
Lee, SH ;
Hwang, SB ;
Park, KY .
PLANT JOURNAL, 2000, 24 (01) :45-56