Development of a Method for Detecting Coxiella burnetii in Cheese Samples

被引：17

作者：

Hirai, Akihiko ^{[1
]}

Nakama, Akiko ^{[1
]}

Chiba, Takashi ^{[1
]}

Kai, Akemi ^{[1
]}

机构：

[1] Tokyo Metropolitan Inst Publ Hlth, Dept Microbiol, Shinjyuku Ku, Tokyo 1690073, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2012年 / 74卷 / 02期

关键词：

cheese; contamination; Coxiella burnetii; nested PCR; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; Q-FEVER; DAIRY-PRODUCTS; MILK; CHILDREN; BOVINE; JAPAN; ASSAY; COWS;

D O I：

10.1292/jvms.11-0023

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Coxiella burnetii is the causative agent of Q fever, and the main route of infection in humans is inhalation of contaminated aerosols. Although oral transmission by contaminated raw milk or dairy products is also a possible route of human infection, there have been few studies investigating the presence of C. burnetii in dairy products. We developed a new method of extracting DNA from cheese and detecting C. burnetii DNA in cheese samples with a nested PCR assay. The limit of detection was 6.0 x 10(2) C. burnetii particles per gram. We subsequently used this method to examine the presence of C. burnetii in cheese at commercial markets in Tokyo from June 2005 to December 2008. Twenty-eight of 147 cheese samples were found to be positive for C. burnetii DNA. However, when we assessed the viability of C. burnetii by inoculating mice with DNA-positive samples, all of the samples were found to be negative. Thus, the viability of C. burnetii appears to have been lost in these cheese samples.

引用

页码：175 / 180

页数：6

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