Cell-free DNA in the urine of rats exposed to ionizing radiation

被引：7

作者：

Abdullaev, Serazhutdin A. ^{[1
]}

Minkabirova, Gulchachak M. ^{[1
,2
]}

Bezlepkin, Vladimir G. ^{[1
]}

Gaziev, Azhub I. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Theoret & Expt Biophys, Pushchino 142290, Moscow Region, Russia

[2] Pushchino State Inst Nat Sci, Pushchino 142290, Moscow Region, Russia

来源：

RADIATION AND ENVIRONMENTAL BIOPHYSICS | 2015年 / 54卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

Urine mitochondrial and nuclear DNA; Irradiated rats; Real-time PCR; MOLECULAR-WEIGHT DNA; MITOCHONDRIAL-DNA; NUCLEIC-ACIDS; FETAL DNA; PLASMA; CANCER; MUTATIONS; QUANTIFICATION; REPLICATION; CIRCULATION;

D O I：

10.1007/s00411-015-0599-0

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Investigation of cell-free DNA (cf-DNA) in body fluids, as a potential biomarker for assessing the effect of ionizing radiation on the organism, is of considerable interest. We investigated changes in the contents of cell-free mitochondrial DNA (cf-mtDNA) and cell-free nuclear DNA (cf-nDNA) in the urine of X-ray-exposed rats. Assays of cf-mtDNA and cf-nDNA were performed by a real-time PCR in rat urine collected before and after irradiation of animals with doses of 3 and 5 Gy. We also determined the presence of mutations in urine cf-mtDNA, as recognized by Surveyor nuclease. A sharp increase in cf-mtDNA and cf-nDNA in the urine of irradiated rats was observed within 24 h after exposure, followed by a decrease to normal levels. In all cases, the contents of cf-mtDNA fragment copies (estimated by gene tRNA) were significantly higher than those of cf-nDNA estimated by gene GAPDH. A certain portion of mutant cf-mtDNA fragments was detected in the urine of exposed rats, whereas they were absent in the urine of the same animals before irradiation. These preliminary data also suggest that the increased levels of urine cf-mtDNA and cf-nDNA may be a potential biomarker for noninvasive assessment of how the organism responds to ionizing radiation influence.

引用

页码：297 / 304

页数：8

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