Effect of cationic liposome composition on in vitro cytotoxicity and protective effect on carried DNA

被引：97

作者：

Cortesi, R

Esposito, E

Menegatti, E

Gambari, R

Nastruzzi, C

机构：

[1] UNIV FERRARA,DIPARTIMENTO SCI FARMACEUT,I-44100 FERRARA,ITALY

[2] UNIV FERRARA,CTR BIOTECHNOL,I-44100 FERRARA,ITALY

[3] UNIV NOTTINGHAM,DEPT PHARMACEUT SCI,NOTTINGHAM NG7 2RD,ENGLAND

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 1996年 / 139卷 / 1-2期

关键词：

cationic liposomes; DNA stability; cytotoxicity;

D O I：

10.1016/0378-5173(96)04574-7

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Positively charged liposomes were prepared by using three different cationic surfactants, namely cetyl-trimethyl-ammonium bromide (CTAB), didodecyl-dimethyl-ammonium bromide (DDAB(12)) and dioctadecyl-dimethyl-ammonium bromide (DDAB(18)). A study of the parameters influencing the in vitro toxicity of cationic liposomes on cultured cell lines was performed, demonstrating the lower cytotoxicity of DDAB(18)-containing liposomes. In addition, the stability of lambda DNA complexed to cationic liposomes after exposure to serum exo- and endonucleases was evaluated. Our results indicated that DDAB(12) and DDAB(18) liposomes are able to efficiently protect DNA from degradation, thus representing a potential approach to deliver nucleic acid in vivo.

引用

页码：69 / 78

页数：10

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