The dynamic changes of Th17/Treg cytokines in rat liver transplant rejection and tolerance

Acute rejection is still a major cause of early graft loss and a risk factor for long-term recipient post-transplant survival. Recently, CD4(+)CD25(+)Foxp3(+) regulatory T (Treg) cells and Th17 cells have been described as two distinct subsets with opposing effects on autoimmunity and transplant immunity. We investigated the existence of Th17/Treg functional imbalance between tolerance and rejection groups during rat liver transplantation. Then, Th17/Treg functions on different levels were investigated comparatively between those two groups, including related cytokine secretion and key transcription factors. REJ groups revealed significant increase in Th17-related cytokine (IL-17, IL-6 and IL-23) and transcription factor (ROR gamma t) levels and remarkable decrease in Treg-related cytokine (IL-10 and TGF-beta 1) and transcription factor (Foxp3) levels when compared to day-matched TOL groups from day 3 post-transplantation. Results indicated Th17/Treg functional imbalance between tolerance and rejection groups during rat liver transplantation, suggesting a potential role of Th17/Treg imbalance in pathogenesis of acute transplant rejection. (C) 2011 Elsevier B.V. All rights reserved.