Two isoforms of chicken melanopsins show blue light sensitivity

被引：73

作者：

Torii, Masaki ^{[1
]}

Kojima, Daisuke ^{[1
]}

Okano, Toshiyuki ^{[1
]}

Nakamura, Atsushi ^{[1
]}

Terakita, Akihisa ^{[2
]}

Shichida, Yoshinori ^{[2
]}

Wada, Akimori ^{[3
]}

Fukada, Yoshitaka ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Sci, Dept Biophys & Biochem, Bunkyo Ku, Tokyo 1130033, Japan

[2] Kyoto Univ, Grad Sch Sci, Dept Biophys, Kyoto 6068502, Japan

[3] Kobe Pharmaceut Univ, Dept Organ Chem Life Sci, Higashinada Ku, Kobe, Hyogo 6588558, Japan

来源：

FEBS LETTERS | 2007年 / 581卷 / 27期

关键词：

melanopsin; OPN4; pineal gland;

D O I：

10.1016/j.febslet.2007.10.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Melanopsin is a vertebrate non-visual opsin and functions as a circadian photoreceptor in mammalian retinas. Here we found the expression of two kinds of melanopsin genes in the chicken pineal gland and identified the presence of five isoforms derived from these two genes. Reconstitution of the recombinant proteins with 11-cis-retinal revealed that at least two of these melanopsin protein isoforms can function as blue-sensitive photopigments with absorption maxima at 476-484 nm. These values are consistent with maximal sensitivities of action spectra determined from the physiological and behavioral studies on mammalian melanopsins. The melanopsin isoforms found in this study may function as pineal circadian photoreceptors. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：5327 / 5331

页数：5

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