The development of a nucleus staining fluorescent probe for dynamic mitosis imaging in live cells

被引：13

作者：

Ghosh, Krishna Kanta ^{[1
,2
]}

Jeong, Yun-Mi ^{[3
]}

Kang, Nam-Young ^{[3
]}

Lee, JungYeol ^{[1
,2
]}

Diana, Wan Si Yan ^{[3
]}

Kim, Jun-Young ^{[3
]}

Yoo, Jaeduk ^{[1
,2
]}

Kim, Dohee ^{[4
,5
]}

Kim, Yun Kyung ^{[4
,6
]}

Chang, Young-Tae ^{[1
,2
,3
]}

机构：

[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

[2] Natl Univ Singapore, MedChem Program, Inst Life Sci, Singapore 117543, Singapore

[3] ASTAR, Singapore Bioimaging Consortium, Lab Bioimaging Probe Dev, Singapore 138667, Singapore

[4] Korea Inst Sci & Technol, Ctr Neuromed, Brain Sci Inst, Seoul, South Korea

[5] Yonsei Univ, Dept Biotechnol, Coll Life Sci & Biotechnol, Translat Res Ctr Prot Funct Control, Seoul 120749, South Korea

[6] Univ Sci & Technol, Dept Biol Chem, Daejon, South Korea

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 45期

关键词：

CLICK CHEMISTRY; CYTO-TOXICITY; LIVING CELLS; CYCLE; HOECHST-33342; CYCLOADDITION; VISUALIZATION; DIVISION; LIBRARY;

D O I：

10.1039/c5cc02295a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A low-toxicity nucleus staining fluorescent probe, CDb12, was developed for real time mitosis imaging in live cells. CDb12 was identified by unbiased high-throughput imaging-based screening of a new xanthone library (AX). Unlike the conventional Hoechst dye, the low toxicity of CDb12 allows long term monitoring of cell division over more than one cell cycle.

引用

页码：9336 / 9338

页数：3

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