Antibacterial activity of chitosan/collagen membranes containing red propolis extract

被引:11
作者
Loureiro, K. Cavalcante [1 ,2 ]
Barbosa, T. Carvalho [2 ]
Nery, M. [2 ]
Chaud, M. Vinicius [3 ]
Silva, C. Ferreira [4 ]
da Andrade, L. Nalone [5 ]
Correa, C. Bani [5 ]
Jaguer, A. [6 ]
Padilha, F. Ferreira [1 ,2 ]
Cardoso, J. Cordeiro [1 ,2 ]
Souto, E. [7 ,8 ]
Severino, P. [1 ,2 ,9 ]
机构
[1] Inst Technol & Res ITP, Aracaju, Brazil
[2] Tiradentes Univ UNIT, Aracaju, Brazil
[3] Sorocaba Univ UNISO, Dept Technol & Environm Proc, Sorocaba, Brazil
[4] Fed Univ Sao Paulo UNIFESP, Dept Exact Sci & Earth, Diadema, Brazil
[5] Fed Univ Sergipe UFS, Sao Cristovao, Brazil
[6] Inst Macromol Chem, Prague, Czech Republic
[7] Univ Coimbra FFUC, Dept Pharmaceut Technol, Fac Pharm, Coimbra, Portugal
[8] Univ Minho, Ctr Biol Engn CEB, Fac Pharm, Braga, Portugal
[9] Tiradentes Inst, Dorchester, MA USA
来源
PHARMAZIE | 2020年 / 75卷 / 2-3期
关键词
BACCHARIS-DRACUNCULIFOLIA; BOTANICAL ORIGIN; COMPOSITE FILMS; CHITOSAN; ANTIOXIDANT;
D O I
10.1691/ph.2020.9050
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
In this study we developed a mucoadhesive polymeric membrane wound dressing incorporating red propolis extract (HERP). Membranes were made using a casting method employing collagen, chitosan, polyethylene glycol (15, 20, and 30v%), and hydroethanolic extract of EtOH-H2O 70v% - 30v% (v/v) of HERP (0.5, 1.0, and 1.5%). Membranes were extensively characterized to assess the thickness, pH, morphology using Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC), mechanical properties, swelling, in vitro mucoadhesion, cytotoxicity, and minimum inhibitory concentration (MIC). Assessment of the thickness and mechanical properties of the membranes containing HERP revealed that the most significant thickness obtained was 40.7 mu m; thermal analysis suggests suggesting the hydrogen bonds between hydroxyl groups of isoflavones and the free amine present in the region of chitosan. Cell viability decreased as the amount of HERP increased. Finally, the MICs were 7.8 and 1.9 mu g.mL-1 for Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853, respectively. These results were suggesting that the 0.5 % HERP membrane has the potential for future studies for wound application.
引用
收藏
页码:75 / 81
页数:7
相关论文
共 45 条
[1]  
Andrews GP, 2015, J PHARM SCI-US, V104, P4329, DOI [10.1002/JPS.24679, 10.1002/jps.24679]
[2]   Development of antimicrobial and scar preventive chitosan hydrogel wound dressings [J].
Anjum, Sadiya ;
Arora, Abha ;
Alam, M. S. ;
Gupta, Bhuvanesh .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2016, 508 (1-2) :92-101
[3]   Design and characterization of chitosan/zeolite composite films - Effect of zeolite type and zeolite dose on the film properties [J].
Barbosa, Gustavo P. ;
Debone, Henrique S. ;
Severino, Patricia ;
Souto, Eliana B. ;
da Silva, Classius F. .
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2016, 60 :246-254
[4]   Brazilian Red Propolis Induces Apoptosis-Like Cell Death and Decreases Migration Potential in Bladder Cancer Cells [J].
Begnini, Karine Rech ;
Moura de Leon, Priscila Marques ;
Thurow, Helena ;
Schultze, Eduarda ;
Campos, Vinicius Farias ;
Rodrigues, Fernanda Martins ;
Borsuk, Sibele ;
Dellagostin, Odir Antonio ;
Savegnago, Lucielli ;
Roesch-Ely, Mariana ;
Moura, Sidnei ;
Padilha, Francine F. ;
Collares, Tiago ;
Pegas Henriques, Joao Antonio ;
Seixas, Fabiana Koemmling .
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2014, 2014
[5]   Coupling tyrosol, quercetin or ferulic acid and electron beam irradiation to cross-link chitosan-gelatin films: A structure-function approach [J].
Benbettaieb, Nasreddine ;
Karbowiak, Thomas ;
Brachais, Claire-Helene ;
Debeaufort, Frederic .
EUROPEAN POLYMER JOURNAL, 2015, 67 :113-127
[6]   Properties of gelatin-based films with added ethanol-propolis extract [J].
Bodini, R. B. ;
Sobral, P. J. A. ;
Favaro-Trindade, C. S. ;
Carvalho, R. A. .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2013, 51 (01) :104-110
[7]   Oral films: Current status and future perspectives I - Galenical development and quality attributes [J].
Borges, Ana Filipa ;
Silva, Claudia ;
Coelho, Jorge F. J. ;
Simoes, Sergio .
JOURNAL OF CONTROLLED RELEASE, 2015, 206 :1-19
[8]  
BRASIL, 2001, PEC AB INSTR NORM N
[9]   Antioxidant and antimicrobial edible zein/chitosan composite films fabricated by incorporation of phenolic compounds and dicarboxylic acids [J].
Cheng, Siang-Ying ;
Wang, Be-Jen ;
Weng, Yih-Ming .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2015, 63 (01) :115-121
[10]   The preparation, characterization and evaluation of regenerated cellulose/collagen composite hydrogel films [J].
Cheng, Yongmei ;
Lu, Jinting ;
Liu, Shilin ;
Zhao, Peng ;
Lu, Guozhong ;
Chen, Jinghua .
CARBOHYDRATE POLYMERS, 2014, 107 :57-64