Ethanol perturbs lipid organization in models of stratum corneum membranes: An investigation combining differential scanning calorimetry, infrared and 2H NMR spectroscopy

被引：50

作者：

Kwak, Sungjong ^{[1
]}

Brief, Elana ^{[2
]}

Langlais, Denis ^{[2
]}

Kitson, Neil ^{[4
]}

Lafleur, Michel ^{[1
]}

Thewalt, Jenifer ^{[2
,3
,4
]}

机构：

[1] Univ Montreal, Dept Chem, CSACS, Montreal, PQ H3C 3J7, Canada

[2] Simon Fraser Univ, Dept Phys, Burnaby, BC V5A 1S6, Canada

[3] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada

[4] Univ British Columbia, Dept Dermatol & Skin Sci, Vancouver, BC V5Z 4E8, Canada

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2012年 / 1818卷 / 05期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Ethanol; H-2; NMR; IR; DSC; Ceramide; Stratum corneum; EPIDERMAL PERMEABILITY BARRIER; NUCLEAR-MAGNETIC-RESONANCE; X-RAY-DIFFRACTION; PHASE-BEHAVIOR; HUMAN SKIN; FATTY-ACIDS; IN-VITRO; INTERCELLULAR MEMBRANES; PERCUTANEOUS-ABSORPTION; PERMEATION ENHANCEMENT;

D O I：

10.1016/j.bbamem.2012.02.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ethanol is used in a variety of topical products. It is known to enhance the permeability of the skin by altering the ability of the stratum corneum (SC) intercellular membranes to form an effective barrier. In addition, ethanol and other alcohols are key components of antiseptic gels currently used for hand wash. Using infrared and deuterium NMR spectroscopy as well as calorimetry, we have investigated the effect of ethanol on a model membrane composed of lipids representing the three classes of SC lipids, an equimolar mixture of N-palmitoylsphingosine (ceramide), palmitic acid and cholesterol. Ethanol is found to influence the membrane in a dose dependent manner, disrupting packing and increasing lipid motion at low concentrations and selectively extracting lipids at moderate concentrations. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：1410 / 1419

页数：10

共 61 条

[1] EXAMINATION OF THE EFFECT OF ETHANOL ON HUMAN STRATUM-CORNEUM INVIVO USING INFRARED-SPECTROSCOPY [J].

BOMMANNAN, D ;

POTTS, RO ;

GUY, RH .

JOURNAL OF CONTROLLED RELEASE, 1991, 16 (03) :299-304

[2]

Bouwstra JA, 1996, J LIPID RES, V37, P999

[3] STRUCTURE OF HUMAN STRATUM-CORNEUM AS A FUNCTION OF TEMPERATURE AND HYDRATION - A WIDE-ANGLE X-RAY-DIFFRACTION STUDY [J].

BOUWSTRA, JA ;

GOORIS, GS ;

SALOMONSDEVRIES, MA ;

VANDERSPEK, JA ;

BRAS, W .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1992, 84 (03) :205-216

[4] STRUCTURAL INVESTIGATIONS OF HUMAN STRATUM-CORNEUM BY SMALL-ANGLE X-RAY-SCATTERING [J].

BOUWSTRA, JA ;

GOORIS, GS ;

VANDERSPEK, JA ;

BRAS, W .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1991, 97 (06) :1005-1012

[5]

BOUWSTRA JA, 1995, J LIPID RES, V36, P685

[6] Phase Behavior of an Equimolar Mixture of N-Palmitoyl-D-erythro-sphingosine, Cholesterol, and Palmitic Acid, a Mixture with Optimized Hydrophobic Matching [J].

Brief, Elana ;

Kwak, Sungjong ;

Cheng, John T. J. ;

Kitson, Neil ;

Thewalt, Jenifer ;

Lafleur, Michel .

LANGMUIR, 2009, 25 (13) :7523-7532

[7] DUAL-CONTROLLED TRANSDERMAL DELIVERY OF LEVONORGESTREL AND ESTRADIOL - ENHANCED PERMEATION AND MODULATED DELIVERY [J].

CHEN, GS ;

KIM, DD ;

CHIEN, YW .

JOURNAL OF CONTROLLED RELEASE, 1995, 34 (02) :129-143

[8] Fourier transform infrared spectroscopy and differential scanning calorimetry studies of fatty acid homogeneous ceramide 2 [J].

Chen, HC ;

Mendelsohn, R ;

Rerek, ME ;

Moore, DJ .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2000, 1468 (1-2) :293-303

[9] Effect of cholesterol on miscibility and phase behavior in binary mixtures with synthetic ceramide 2 and octadecanoic acid. Infrared studies [J].

Chen, HC ;

Mendelsohn, R ;

Rerek, ME ;

Moore, DJ .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2001, 1512 (02) :345-356

[10] Human stratum corneum lipids have a distorted orthorhombic packing at the surface of cohesive failure [J].

Chen, TL ;

Wiedmann, TS .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1996, 107 (01) :15-19

← 1 2 3 4 5 6 7 →