Pigment Epithelium-Derived Factor Released by Muller Glial Cells Exerts Neuroprotective Effects on Retinal Ganglion Cells

被引:54
作者
Unterlauft, Jan Darius [1 ,2 ]
Eichler, Wolfram [1 ,2 ]
Kuhne, Konstantin [1 ,2 ]
Yang, Xiu Mei [1 ,2 ]
Yafai, Yousef [1 ,2 ]
Wiedemann, Peter [1 ,2 ]
Reichenbach, Andreas [3 ]
Claudepierre, Thomas [1 ,2 ]
机构
[1] Univ Leipzig, Dept Ophthalmol, D-04103 Leipzig, Germany
[2] Univ Leipzig, Hosp Eye, D-04103 Leipzig, Germany
[3] Univ Leipzig, Paul Flechsig Inst Brain Res, D-04109 Leipzig, Germany
关键词
Neuroprotection; Neuroregeneration; Neuron-glia interaction; Reactive glia; GLUTAMINE-SYNTHETASE EXPRESSION; ANTIINFLAMMATORY FACTOR; ISCHEMIC-INJURY; DBA/2J MICE; PEDF; GLAUCOMA; SURVIVAL; HYPOXIA; MOUSE; NEOVASCULARIZATION;
D O I
10.1007/s11064-012-0747-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Survival of retinal ganglion cells (RGC) is compromised in several vision-threatening disorders such as ischemic and hypertensive retinopathies and glaucoma. Pigment epithelium-derived factor (PEDF) is a naturally occurring pleiotropic secreted factor in the retina. PEDF produced by retinal glial (Muller) cells is suspected to be an essential component of neuron-glial interactions especially for RGC, as it can protect this neuronal type from ischemia-induced cell death. Here we show that PEDF treatment can directly affect RGC survival in vitro. Using Muller cell-RGC-co-cultures we observed that activity of Muller-cell derived soluble mediators can attenuate hypoxia-induced damage and RGC loss. Finally, neutralizing the activity of PEDF in glia-conditioned media partially abolished the neuroprotective effect of glia, leading to an increased neuronal death in hypoxic condition. Altogether our results suggest that PEDF is crucially involved in the neuroprotective process of reactive Muller cells towards RGC.
引用
收藏
页码:1524 / 1533
页数:10
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