Probing lectin and sperm with carbohydrate-modified quantum dots

被引：77

作者：

Robinson, A

Fang, JM ^{[1
]}

Chou, PT

Liao, KW

Chu, RM

Lee, SJ

机构：

[1] Natl Taiwan Univ, Dept Chem, Taipei 106, Taiwan

[2] Natl Taiwan Univ, Dept Vet Med, Taipei 106, Taiwan

[3] Natl Taiwan Univ, Dept Life Sci, Taipei 106, Taiwan

[4] Natl Taiwan Univ, Inst Zool, Taipei 106, Taiwan

来源：

CHEMBIOCHEM | 2005年 / 6卷 / 10期

关键词：

biosensors; fluorescent probes; glycosides; molecular recognition; nanotechnology;

D O I：

10.1002/cbic.200500112

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the encapsulation of quantum dots with biologically important beta-N-acetylglucosamine (GlcNAc) in different ratios, together with studies of their specific/sensitive multivalent interactions with lectins and sperm by fluorimetry, transmission electron microscopy, dynamic light scattering microscopy, confocal imaging techniques, and flow cytometry. These GlcNAc-encapsulated quantum dots (QDGLNs) specifically bind to wheat germ agglutinin, and cause fluorescence quenching and aggregation. Further studies of QDGLNs and the mannose-encapsulated QDs (QDMANs) with sperm revealed site-specific interactions, in which QDGLNs bind to the head of the sperm, while QDMANs spread over the whole sperm body.

引用

页码：1899 / 1905

页数：7

共 86 条

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