Role of commercially available SARS-CoV-2 detection kits in pandemic of COVID-19 on the basis of N and E gene detection

被引:0
作者
Khalid, Mubeen [1 ]
Shear, Rani Wafa [1 ]
Rehman, Wadiat [1 ]
Rehman, Rahat [1 ]
Ali, Babar [2 ]
Nazir, Shahid [1 ]
Basharat, Muhammad Usman [3 ]
Khan, Muhammad Farhan [1 ,4 ]
机构
[1] Univ Hlth Sci Lahore Pakistan, Dept Forens Sci, Lahore, Pakistan
[2] Khawaja Fareed Univ Engn & Informat Technol, Dept Biosci & Technol, Ry Khan, Pakistan
[3] Primary & Secondary Healthcare Dept, Lahore, Pakistan
[4] Alkhidmat Hlth Fdn Pakistan, Alkhidmat diagnost, Lahore, Pakistan
来源
ADVANCEMENTS IN LIFE SCIENCES | 2021年 / 8卷 / 04期
关键词
Role of commercially available kits; SARS-CoV2; Pandemic of Covid-19; N gene; E gene; RESPIRATORY SYNDROME CORONAVIRUS; RNA-POLYMERASE; PROTEIN; IDENTIFICATION; SPIKE; PCR;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Coronavirus has blowout worldwide from the time when its revelation in Hubei Province, China in December 2019 introducing a genuine general wellbeing emergency. The capacity to recognize an irresistible specialist in a broad pestilence is vital to the achievement of isolate endeavors notwithstanding the delicate and precise screening of expected instances of disease from patients in a clinical setting. Structural proteins the basic key role-playing in SARS-CoV2 identification include a spike, envelope membrane, nucleocapsid, and helper proteins. N-protein ties to the infection single positive-strand RNA that permits the infection to assume control over human cells and transform them into infection industrial facilities inside the capsid and E-protein shows a significant part in infection gathering, film permeability of the host cell, and infection has cell correspondence. Nucleic-Acid base testing presently offers the most touchy and early discovery of COVID-19. Notwithstanding, analytic advancements have explicit impediments and announced a few false negative and false positive cases, particularly during the beginning phases of contamination. Presently, more refined diagnostics are being created to improve the COVID-19 determination. This article presents an outline of diagnostic approaches to address a few inquiries and issues identified with the constraints of flow innovations and future innovative work difficulties to empower ideal, fast, minimal effort, and precise analysis of arising irresistible illnesses We depict purpose of-care diagnostics that are not too far off and urge scholastics to propel their advancements past origination. Creating fitting and-play diagnostics to deal with the SARS-CoV-2 flare-up would be valuable in forestalling forthcoming pandemics.
引用
收藏
页码:320 / 325
页数:6
相关论文
共 40 条
  • [1] Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia
    Chu, Daniel K. W.
    Pan, Yang
    Cheng, Samuel M. S.
    Hui, Kenrie P. Y.
    Krishnan, Pavithra
    Liu, Yingzhi
    Ng, Daisy Y. M.
    Wan, Carrie K. C.
    Yang, Peng
    Wang, Quanyi
    Peiris, Malik
    Poon, Leo L. M.
    [J]. CLINICAL CHEMISTRY, 2020, 66 (04) : 549 - 555
  • [2] Corman V., 2020, Diagnostic detection of Wuhan coronavirus 2019 by real-time RT-PCR, P13
  • [3] Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR (Publication with Expression of Concern)
    Corman, Victor M.
    Landt, Olfert
    Kaiser, Marco
    Molenkamp, Richard
    Meijer, Adam
    Chu, Daniel K. W.
    Bleicker, Tobias
    Bruenink, Sebastian
    Schneider, Julia
    Schmidt, Marie Luisa
    Mulders, Daphne G. J. C.
    Haagmans, Bart L.
    van der Veer, Bas
    van den Brink, Sharon
    Wijsman, Lisa
    Goderski, Gabriel
    Romette, Jean-Louis
    Ellis, Joanna
    Zambon, Maria
    Peiris, Malik
    Goossens, Herman
    Reusken, Chantal
    Koopmans, Marion P. G.
    Drosten, Christian
    [J]. EUROSURVEILLANCE, 2020, 25 (03) : 23 - 30
  • [4] Craw P, 2012, LAB CHIP, V12, P2469, DOI [10.1039/c2lc40100b, 10.1039/c21c40100b]
  • [5] Origin and evolution of pathogenic coronaviruses
    Cui, Jie
    Li, Fang
    Shi, Zheng-Li
    [J]. NATURE REVIEWS MICROBIOLOGY, 2019, 17 (03) : 181 - 192
  • [6] A severe acute respiratory syndrome coronavirus that lacks the E gene is attenuated in vitro and in vivo
    DeDiego, Marta L.
    Alvarez, Enrique
    Almazan, Fernando
    Rejas, Maria Teresa
    Lamirande, Elaine
    Roberts, Anjeanette
    Shieh, Wun-Ju
    Zaki, Sherif R.
    Subbarao, Kanta
    Enjuanes, Luis
    [J]. JOURNAL OF VIROLOGY, 2007, 81 (04) : 1701 - 1713
  • [7] Identification of a novel coronavirus in patients with severe acute respiratory syndrome
    Drosten, C
    Günther, S
    Preiser, W
    van der Werf, S
    Brodt, HR
    Becker, S
    Rabenau, H
    Panning, M
    Kolesnikova, L
    Fouchier, RAM
    Berger, A
    Burguière, AM
    Cinatl, J
    Eickmann, M
    Escriou, N
    Grywna, K
    Kramme, S
    Manuguerra, JC
    Müller, S
    Rickerts, V
    Stürmer, M
    Vieth, S
    Klenk, HD
    Osterhaus, ADME
    Schmitz, H
    Doerr, HW
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) : 1967 - 1976
  • [8] Bat Coronaviruses in China
    Fan, Yi
    Zhao, Kai
    Shi, Zheng-Li
    Zhou, Peng
    [J]. VIRUSES-BASEL, 2019, 11 (03):
  • [9] Quantitative RT-PCR: Pitfalls and potential
    Freeman, WM
    Walker, SJ
    Vrana, KE
    [J]. BIOTECHNIQUES, 1999, 26 (01) : 112 - +
  • [10] In-silicoapproaches to detect inhibitors of the human severe acute respiratory syndrome coronavirus envelope protein ion channel
    Gupta, Manoj Kumar
    Vemula, Sarojamma
    Donde, Ravindra
    Gouda, Gayatri
    Behera, Lambodar
    Vadde, Ramakrishna
    [J]. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2021, 39 (07) : 2617 - 2627