RNA-targeting CRISPR comes of age

被引：12

作者：

Kim, V. Narry ^{[1
,2
]}

机构：

[1] Inst for Basic Sci Korea, Ctr RNA Res, Seoul, South Korea

[2] Seoul Natl Univ, Sch Biol Sci, Seoul, South Korea

来源：

NATURE BIOTECHNOLOGY | 2018年 / 36卷 / 01期

关键词：

GUIDE-RNA;

D O I：

10.1038/nbt.4054

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cas13 can be harnessed for RNA knockdown and editing in mammalian cells, opening up a range of applications in RNA biology.

引用

页码：44 / 45

页数：3

共 9 条

[1] RNA targeting with CRISPR-Cas13 [J].

Abudayyeh, Omar O. ;

Gootenberg, Jonathan S. ;

Essletzbichler, Patrick ;

Han, Shuo ;

Joung, Julia ;

Belanto, Joseph J. ;

Verdine, Vanessa ;

Cox, David B. T. ;

Kellner, Max J. ;

Regev, Aviv ;

Lander, Eric S. ;

Voytas, Daniel F. ;

Ting, Alice Y. ;

Zhang, Feng .

NATURE, 2017, 550 (7675) :280-+

[2] C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector [J].

Abudayyeh, Omar O. ;

Gootenberg, Jonathan S. ;

Konermann, Silvana ;

Joung, Julia ;

Slaymaker, Ian M. ;

Cox, David B. T. ;

Shmakov, Sergey ;

Makarova, Kira S. ;

Semenova, Ekaterina ;

Minakhin, Leonid ;

Severinov, Konstantin ;

Regev, Aviv ;

Lander, Eric S. ;

Koonin, Eugene V. ;

Zhang, Feng .

SCIENCE, 2016, 353 (6299)

[3] RNA editing with CRISPR-Cas13 [J].

Cox, David B. T. ;

Gootenberg, Jonathan S. ;

Abudayyeh, Omar O. ;

Franklin, Brian ;

Kellner, Max J. ;

Joung, Julia ;

Zhang, Feng .

SCIENCE, 2017, 358 (6366) :1019-1027

[4] Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection [J].

East-Seletsky, Alexandra ;

O'Connell, Mitchell R. ;

Knight, Spencer C. ;

Burstein, David ;

Cate, Jamie H. D. ;

Tjian, Robert ;

Doudna, Jennifer A. .

NATURE, 2016, 538 (7624) :270-+

[5] Construction of a guide-RNA for site-directed RNA mutagenesis utilising intracellular A-to-I RNA editing [J].

Fukuda, Masatora ;

Umeno, Hiromitsu ;

Nose, Kanako ;

Nishitarumizu, Azusa ;

Noguchi, Ryoma ;

Nakagawa, Hiroyuki .

SCIENTIFIC REPORTS, 2017, 7

[6] Nucleic acid detection with CRISPR-Cas13a/C2c2 [J].

Gootenberg, Jonathan S. ;

Abudayyeh, Omar O. ;

Lee, Jeong Wook ;

Essletzbichler, Patrick ;

Dy, Aaron J. ;

Joung, Julia ;

Verdine, Vanessa ;

Donghia, Nina ;

Daringer, Nichole M. ;

Freije, Catherine A. ;

Myhrvold, Cameron ;

Bhattacharyya, Roby P. ;

Livny, Jonathan ;

Regev, Aviv ;

Koonin, Eugene V. ;

Hung, Deborah T. ;

Sabeti, Pardis C. ;

Collins, James J. ;

Zhang, Feng .

SCIENCE, 2017, 356 (6336) :438-+

[7] Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme [J].

Knott, Gavin J. ;

East-Seletsky, Alexandra ;

Cofsky, Joshua C. ;

Holton, James M. ;

Charles, Emeric ;

O'Connell, Mitchell R. ;

Doudna, Jennifer A. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2017, 24 (10) :825-+

[8] Mechanistic insights into editing-site specificity of ADARs [J].

Kuttan, Ashani ;

Bass, Brenda L. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (48) :E3295-E3304

[9] Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities [J].

Liu, Liang ;

Li, Xueyan ;

Wang, Jiuyu ;

Wang, Min ;

Chen, Peng ;

Yin, Maolu ;

Li, Jiazhi ;

Sheng, Gang ;

Wang, Yanli .

CELL, 2017, 168 (1-2) :121-+

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