Toxoplasma gondii: Pig seroprevalence, associated risk factors and viability in fresh pork meat

被引:38
作者
Herrero, Laura [1 ]
Jesus Gracia, Maria [2 ]
Perez-Arquillue, Consuelo [1 ]
Lazaro, Regina [1 ]
Herrera, Marta [1 ]
Herrera, Antonio [1 ]
Bayarri, Susana [1 ]
机构
[1] Univ Zaragoza, Fac Vet, Inst Agroalimentario Aragon IA2, Dept Prod Anim & Ciencia Alimentos,CITA, C Miguel Servet 177, E-50013 Zaragoza, Spain
[2] Univ Zaragoza, Fac Vet, Inst Agroalimentario Aragon IA2, Dept Patol Anim,CITA, C Miguel Servet 177, E-50013 Zaragoza, Spain
关键词
Toxoplasma gondii; Pig seroprevalence; Risk factors; Pork meat; Safety control tool; HACCP; Cured meat products; POLYMERASE-CHAIN-REACTION; CROSS-SECTIONAL SURVEY; PREGNANT-WOMEN; DOMESTIC PIGS; MOLECULAR CHARACTERIZATION; HIGH PREVALENCE; BREEDING FARMS; SWINE FARMS; CURED HAM; INFECTION;
D O I
10.1016/j.vetpar.2016.05.010
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
This study was conducted on 161 fattening pig farms located in Aragon (Northeast Spain). Serum samples from 1200 pigs were tested for antibodies against T. gondii by indirect immunofluorescence assay (IFA). Antibodies to T. gondii (>= 1:20) were detected in 301 pigs (24.52%). The seroprevalence observed in the present study indicates a widespread exposure to T. gondii, as seropositive pigs were found in 96.67% of the farms studied although low pig titers were determined. Risk factors associated with T. gondii seroprevalence were presence of cats in or around the farms, presence of dogs around the facilities, low number of animals in the farms, poor hygiene and bad maintenance of the farms. Finally, it was observed that where rodent baits were used, Toxoplasma prevalence was lower. Risk management measures including control of cats and rodents on the farms, among others, could help to reduce the observed prevalence levels. By mouse bioassay, T. gondii was detected in 73.7% and isolated from 42.1% of seropositive pigs and a significant relation between the titers of pigs and the presence and viability of T. gondii in the tissues was found. The detection of T. gondii is not possible by currently practiced meat inspection. Nevertheless, the increased probability of detecting viable forms of T. gondii in tissues of pigs with titers >= 1: 80 could be used as the cutoff for discriminating higher risk animals, and could be used as an effective control tool for the industry of cured meat products. In practical terms, we propose that this value could be used as a critical limit in the HACCP system. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:52 / 59
页数:8
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