Discrimination of haloarchaeal genera using Raman spectroscopy and robust methods for multivariate data analysis

被引：11

作者：

Dina, Nicoleta Elena ^{[1
]}

Les, Anda ^{[2
]}

Baricz, Andreea ^{[3
,4
]}

Szoke-Nagy, Tiberiu ^{[1
,4
]}

Leopold, Nicolae ^{[2
]}

Sarbu, Costel ^{[5
]}

Banciu, Horia Leonard ^{[4
,6
]}

机构：

[1] Natl Inst Res & Dev Isotop & Mol Technol, Dept Mol & Biomol Phys, 67-103 Donat, Cluj Napoca 400293, Romania

[2] Babes Bolyai Univ, Fac Phys, 1 Kogalniceanu, Cluj Napoca 400084, Romania

[3] Natl Inst Res & Dev Biol Sci NIRDBS, Inst Biol Res, 48 Republ, Cluj Napoca 400015, Romania

[4] Babes Bolyai Univ, Fac Biol & Geol, Dept Mol Biol & Biotechnol, 5-7 Clinicilor, Cluj Napoca 400006, Romania

[5] Babes Bolyai Univ, Fac Chem & Chem Engn, 11 Arany Janos, Cluj Napoca 400028, Romania

[6] Babes Bolyai Univ, Inst Interdisciplinary Res Bionanosci, Ctr Mol Biol, 42 Treboniu Laurian, Cluj Napoca 400271, Romania

来源：

JOURNAL OF RAMAN SPECTROSCOPY | 2017年 / 48卷 / 08期

关键词：

bacterioruberin; fuzzy principal component analysis; haloarchaea; linear discriminant analysis; Raman spectroscopy; PRINCIPAL COMPONENT ANALYSIS; HALOPHILIC ARCHAEA; BETA-CAROTENE; ATACAMA DESERT; IDENTIFICATION; BACTERIORUBERIN; HYPERSALINE; SPECTRA; MICROORGANISMS; CHEMOMETRICS;

D O I：

10.1002/jrs.5187

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

In this study, we aimed at developing a rapid and non-destructive method to predict the genus-level taxonomic affiliation of phenotypically similar halophilic archaea of Halobacteria Class. For this purpose, representatives of three widespread and frequently isolated haloarchaeal genera (Halobacterium sp., Haloferax sp. and Halorubrum sp.) were investigated by means of Raman spectroscopy. The most relevant Raman bands exhibited at 1505, 1150 and 1000 cm (-1), respectively, were ascribed to the bacterioruberin carotenoid found in haloarchaea. Due to the similar spectral profiles, a robust chemometric approach based on fuzzy principal component analysis and linear discriminant analysis for the classification of the three halophilic strains was employed, and the high-accuracy grouping of spectral data corresponding to those strains was achieved. Copyright (C) 2017 John Wiley & Sons, Ltd.

引用

页码：1122 / 1126

页数：5

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