Development and evaluation of nucleoprotein-based rapid detection test for Siniperca chuatsi rhabdovirus

被引：5

作者：

Chen, Zhixin ^{[1
,2
]}

Gong, Nanxin ^{[2
]}

Niu, Jingxuan ^{[2
]}

Xu, Ruixian ^{[2
]}

Yi, Huashan ^{[3
]}

Song, Yuzhu ^{[2
]}

Han, Qinqin ^{[2
]}

Xia, Xueshan ^{[2
]}

Li, Ningqiu ^{[1
]}

Liu, Fugui ^{[4
]}

Liang, Hongru ^{[1
]}

Zhang, Jinyang ^{[2
]}

机构：

[1] Chinese Acad Fishery Sci, Key Lab Fishery Drug Dev, Minist Agr & Rural Affairs, Key Lab Aquat Anim Immune Technol,Pearl River Fis, Guangzhou 510380, Peoples R China

[2] Kunming Univ Sci & Technol, Fac Life Sci & Technol, Res Ctr Mol Med Yunnan Prov, Kunming 650500, Yunnan, Peoples R China

[3] Southwest Univ, Coll Vet Med, Chongqing 402460, Peoples R China

[4] Yunnan Bo Er Heng Int Biol Pharmaceut Co Ltd, Kunming 650300, Peoples R China

来源：

AQUACULTURE | 2022年 / 546卷

基金：

芬兰科学院;

关键词：

Mandarin fish; Rhabdovirus; Nucleoprotein; Polyclonal antibody; c-ELISA; LINKED-IMMUNOSORBENT-ASSAY; RABIES VIRUS; ANTIBODY; VACCINE;

D O I：

10.1016/j.aquaculture.2021.737403

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Siniperca chuatsi rhabdovirus (SCRV) is a rod- or bullet-shaped negative-stranded RNA virus and a threat to mandarin fish farming. This virus naturally infects fish, such as S. chuatsi and Micropterus salmoides, among others. SCRV-infected fish have a 90% mortality rate. In this study, a recombinant SCRV-N protein and a polyclonal antibody were prepared by gene cloning, constructing the vector of the SCRV nucleoprotein gene, purifying the protein, and immunizing mice with the recombinant nucleoprotein. A SCRV-N competitive enzymelinked immunosorbent assay was established based on the recombinant protein and polyclonal antibody and verified by a large number of experimental fish infected with SCRV. The results confirmed that the method established in this study can be used to confirm whether fish are infected with SCRV with high sensitivity and speed. This rapid detection test is suitable for large-scale applications and will provide support for diagnosing infection with the mandarin fish rhabdovirus.

引用

页数：7

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