Isolation and genetic characterization of H3N8 equine influenza virus from donkeys in China

被引：49

作者：

Qi, Ting ^{[1
]}

Guo, Wei ^{[1
]}

Huang, Wenqiang ^{[1
]}

Dai, Lingli ^{[1
]}

Zhao, Liping ^{[1
]}

Li, Hongmei ^{[1
]}

Li, Xuefeng ^{[1
]}

Zhang, Xinxin ^{[1
]}

Wang, Yingyuan ^{[1
]}

Yan, Yan ^{[1
]}

He, Na ^{[1
]}

Xiang, Wenhua ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Harbin Vet Res Inst, Natl Key Lab Vet Biotechnol, Div Livestock Infect Dis, Harbin 150001, Peoples R China

来源：

VETERINARY MICROBIOLOGY | 2010年 / 144卷 / 3-4期

基金：

黑龙江省自然科学基金;

关键词：

Equine influenza; Donkey; Isolation; Phylogenetic analysis; Prevention; A VIRUS; HEMAGGLUTININ MOLECULE; ANTIGENIC ANALYSIS; EVOLUTION; HORSES; LINEAGES; OUTBREAK; STRAIN;

D O I：

10.1016/j.vetmic.2010.01.006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

During the 2007 outbreak of equine influenza (EIV) in China, an influenza virus designated A/donkey/Xinjiang/5/2007 (donkey/Xinjiang/2007) was isolated from a symptomatic donkey in Xinjiang Uygur Autonomous Region, China. To analyze the genetic evolution of the new isolate, the hemagglutinin (HA) gene of donkey/Xinjiang/2007 was amplified and sequenced. Sequence alignment, prediction of glycosylation sites and phylogenetic analysis of the HAl protein of donkey/Xinjiang/2007 showed most similarity to the Florida sublineage clade 2 of the American lineage of equine influenza viruses. The HAl sequence of donkey/Xinjiang/2007 had high identity with the Chinese EIV strains isolated in recent years and had low identity with A/swine/Anhui/1/2006, a horse-derived influenza virus isolated from pigs in China. Compared with vaccine strains, the HAl amino acid sequence of donkey/Xinjiang/2007 varied in the five putative antigenic sites to different degrees and distributed in a different branch of the HA1 phylogenetic tree. Our findings provide further evidence of an American H3N8 equine influenza virus in China in addition to the European H3N8 equine influenza virus. Accordingly, a vaccine against equine influenza in China should contain components of both strains of viruses to prevent widespread infection. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：455 / 460

页数：6

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