Development and validation of a fluorimetric method to determine curcumin in lipid and polymeric nanocapsule suspensions

被引：25

作者：

Mazzarino, Leticia ^{[1
]}

Bellettini, Ismael Casagrande

Minatti, Edson

Lemos-Senna, Elenara ^{[1
]}

机构：

[1] Univ Fed Santa Catarina, Dept Pharmaceut Sci, Pharmaceut Technol Lab, BR-88040900 Florianopolis, SC, Brazil

来源：

BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2010年 / 46卷 / 02期

关键词：

Curcumin/determination; Fluorimerric method/quantitative analysis; Polymeric nanocapsules/evaluation; Lipid nanocapsules/evaluation; LOADED PLGA NANOPARTICLES; STABILITY; COMPLEXATION; FORMULATION; PACLITAXEL; SOLUBILITY;

D O I：

10.1590/S1984-82502010000200008

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A simple, rapid, and sensitive fluorimetrie method was developed and validated to quantify curcumin in lipid and polymeric nanocapsule suspensions, using acetonitrile as a solvent. The excitation and emission wavelengths were set at 397 am and 508 am, respectively. The calibration graph was linear from 0.1 to 0.6 mu g/mL with a correlation coefficient of 0.9982. The detection and quantitation limits were 0.03 and 0.10 mu g/mL, respectively. The validation results confirmed that the developed method is specific, linear, accurate, and precise for its intended use. The current method was successfully applied to the evaluation of curcumin content in lipid and polymeric nanocapsule suspensions during the early stage of formulation development.

引用

页码：219 / 226

页数：8

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