Determination of endothelin-1 in rats using a high-performance liquid chromatography coupled to electrospray tandem mass spectrometry

被引:9
作者
Walczak, Maria [1 ]
Fedorowicz, Andrzej [2 ]
Chlopicki, Stefan [2 ]
Szymura-Oleksiak, Joanna [1 ]
机构
[1] Jagiellonian Univ, Coll Med, Dept Pharmacokinet & Phys Pharm, PL-30688 Krakow, Poland
[2] Jagiellonian Univ, Coll Med, Dept Expt Pharmacol, Chair Pharmacol, PL-31531 Krakow, Poland
关键词
Endothelin-1; Mass spectrometry; Method validation; Pulmonary hypertension; Rats; INDUCED PULMONARY-HYPERTENSION; NITRIC-OXIDE; NADPH-OXIDASE; VASOCONSTRICTION; BIOANALYSIS; PROGRESSION; VALIDATION; MECHANISMS; EXPRESSION; BIOMARKERS;
D O I
10.1016/j.talanta.2010.05.037
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A sensitive and specific liquid chromatography tandem mass spectrometry method with electrospray ionization for the determination of endothelin-1 in rat plasma and lung effluents has been developed and validated. Detection was achieved by an Applied Biosystems MDS Sciex API 2000 triple quadrupole mass spectrometer coupled to an Agilent 1100 LC system. The limit of detection and the limit of the quantification of ET-1 in matrix buffer was estimated at 40 pM and 1 nM, respectively. The precision and accuracy for both intra- and inter-day determination of the analyte ranged from 2.5% to 14.7% and from 104.2% to 113.3%, respectively. No significant relative matrix effect was observed. Stability of Ef-1 established in a bench-top, autosampler, long-term storage stability as well as freeze/thaw cycles shown no significant degradation products in the samples. The results of the method validation indicated that this method is applicable for the determination of the ET-1 concentration in an effluent from the isolated lung preparation as well as in vivo in plasma samples to evaluate ET-1 as a potential biomarker of the progression of pulmonary endothelial dysfunction and pulmonary hypertension in rats induced by a monocrotaline injection. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:710 / 718
页数:9
相关论文
共 33 条
[1]   CLONING AND EXPRESSION OF A CDNA-ENCODING AN ENDOTHELIN RECEPTOR [J].
ARAI, H ;
HORI, S ;
ARAMORI, I ;
OHKUBO, H ;
NAKANISHI, S .
NATURE, 1990, 348 (6303) :730-732
[2]  
ASHBY MJ, 1995, J CARDIOVASC PHARM, V26, pS247
[3]   Methods for samples preparation in proteomic research [J].
Bodzon-Kulakowska, Anna ;
Bierczynska-Krzysik, Anna ;
Dylag, Tomasz ;
Drabik, Anna ;
Suder, Piotr ;
Noga, Marek ;
Jarzebinska, Justyna ;
Silberring, Jerzy .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 849 (1-2) :1-31
[4]   Endothelial dysfunction in pulmonary hypertension [J].
Budhiraja, R ;
Tuder, RM ;
Hassoun, PM .
CIRCULATION, 2004, 109 (02) :159-165
[5]   Endothelin-I-induced oxidative stress in DOCA-salt hypertension involves NADPH-oxidase-independent mechanisms [J].
Callera, GE ;
Tostes, RC ;
Yogi, A ;
Montezano, ACI ;
Touyz, RM .
CLINICAL SCIENCE, 2006, 110 (02) :243-253
[6]   Isoform-specific quantfication of endothelins in HUVEC culture supernatants by on-line high-performance liquid chromatography/electrospray mass spectrometry [J].
Carrascal, M ;
Schneider, K ;
Peralta, C ;
Escolar, G ;
Gelpi, E ;
Abian, J .
BIOMEDICAL CHROMATOGRAPHY, 2004, 18 (06) :388-395
[7]  
Chlopicki S, 2002, POL J PHARMACOL, V54, P433
[8]   Biomarkers, validation and pharmacokinetic-pharmacodynamic modelling [J].
Colburn, WA ;
Lee, JW .
CLINICAL PHARMACOKINETICS, 2003, 42 (12) :997-1022
[9]   Depletion of the high-abundance plasma proteins [J].
Fountoulakis, M ;
Juranville, JF ;
Jiang, L ;
Avila, D ;
Röder, D ;
Jakob, P ;
Berndt, P ;
Evers, S ;
Langen, H .
AMINO ACIDS, 2004, 27 (3-4) :249-259
[10]   Effect of the sample matrix on the determination of indinavir in human urine by HPLC with turbo ion spray tandem mass spectrometric detection [J].
Fu, I ;
Woolf, EJ ;
Matuszewski, BK .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1998, 18 (03) :347-357