Protection of human γB-crystallin from UV-induced damage by epigallocatechin gallate: spectroscopic and docking studies

被引：18

作者：

Chaudhury, Susmitnarayan ^{[1
]}

Bag, Sudipta ^{[1
]}

Bose, Madhuparna ^{[2
]}

Das, Amit Kumar ^{[2
]}

Ghosh, Ananta Kumar ^{[2
]}

Dasgupta, Swagata ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, Kharagpur 721302, W Bengal, India

[2] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India

来源：

MOLECULAR BIOSYSTEMS | 2016年 / 12卷 / 09期

关键词：

GREEN TEA POLYPHENOLS; AGE-RELATED-CHANGES; HUMAN LENS; TRYPTOPHAN FLUORESCENCE; 2-DIMENSIONAL ELECTROPHORESIS; ULTRAVIOLET-RADIATION; MASS-SPECTROMETRY; SELENITE MODELS; C IRRADIATION; EYE LENS;

D O I：

10.1039/c6mb00256k

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transparency of the human eye lens depends on the solubility and stability of the structural proteins of the eye lens, the crystallins. Although the mechanism of cataract formation is still unclear, it is believed to involve protein misfolding and/or aggregation of proteins due to the influence of several external factors such as ultraviolet (UV) radiation, low pH, temperature and exposure to chemical agents. In this article, we report the study of UV induced photo-damage (under oxidative stress) of recombinant human gamma B-crystallin in vitro in the presence of the major green tea polyphenol, (-)-epigallocatechin gallate (EGCG). We have shown that EGCG has the ability to protect human gamma B-crystallin from oxidative stress-induced photo-damage.

引用

页码：2901 / 2909

页数：9

共 60 条

[1]

Benedek GB, 1997, INVEST OPHTH VIS SCI, V38, P1911

[2] COMPARATIVE AND AGE-DEPENDENT ASPECTS OF CRYSTALLIN SIZE AND DISTRIBUTION IN HUMAN, RABBIT, BOVINE, RAT, CHICKEN, DUCK, FROG AND DOGFISH LENSES [J].

BINDELS, JG ;

BESSEMS, GJJ ;

DEMAN, BM ;

HOENDERS, HJ .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1983, 76 (01) :47-55

[3] Ageing and vision: structure, stability and function of lens crystallins [J].

Bloemendal, H ;

de Jong, W ;

Jaenicke, R ;

Lubsen, NH ;

Slingsby, C ;

Tardieu, A .

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 2004, 86 (03) :407-485

[4] EGCG prevents tryptophan oxidation of cataractous ocular lens human γ-crystallin in presence of H2O2 [J].

Chaudhury, Susmitnarayan ;

Ghosh, Ishita ;

Saha, Gautam ;

Dasgupta, Swagata .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2015, 77 :287-292

[5] Mechanism of the highly efficient quenching of tryptophan fluorescence in human γD-crystallin [J].

Chen, Jiejin ;

Flaugh, Shannon L. ;

Callis, Patrik R. ;

King, Jonathan .

BIOCHEMISTRY, 2006, 45 (38) :11552-11563

[6] Mechanism of the Very Efficient Quenching of Tryptophan Fluorescence in Human γD- and γS-Crystallins: The γ-Crystallin Fold May Have Evolved To Protect Tryptophan Residues from Ultraviolet Photodamage [J].

Chen, Jiejin ;

Callis, Patrik R. ;

King, Jonathan .

BIOCHEMISTRY, 2009, 48 (17) :3708-3716

[7]

CHIOU SH, 1981, J BIOL CHEM, V256, P5176

[8]

Chow HHS, 2003, CLIN CANCER RES, V9, P3312

[9] Photo-oxidation of proteins and its role in cataractogenesis [J].

Davies, MJ ;

Truscott, RJW .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2001, 63 (1-3) :114-125

[10]

de Jong WW., 1981, Molecular and Cellular Biology of the Eye Lens, P221, DOI DOI 10.1146/ANNUREV.ECOLSYS.33.020602.095451

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