Silver <bold>nanoparticles biosynthesised</bold> using Centella asiatica leaf extract: apoptosis induction in MCF-7 breast cancer cell line

被引:40
作者
Fard, Shima Edalat [1 ]
Tafvizi, Farzaneh [1 ]
Torbati, Maryam Bikhof [2 ]
机构
[1] Islamic Azad Univ, Parand Branch, Dept Biol, Parand, Iran
[2] Islamic Azad Univ, Shahr E Rey Branch, Yadegar E Imam Khomeini RAH, Coll Sci,Dept Biol, Tehran, Iran
关键词
ultraviolet spectra; transmission electron microscopy; cellular biophysics; infrared spectra; visible spectra; nanofabrication; cancer; toxicology; nanomedicine; nanoparticles; biomedical materials; scanning electron microscopy; silver; Fourier transform spectra; X-ray diffraction; genetics; enzymes; botany; biochemistry; spherical AgNPs; biosynthesised AgNPs; anti-cancer; apoptosis induction; green synthesised AgNPs; MCF-7 breast cancer cell line; green synthesised silver nanoparticles; Ag; caspase gene expression; flow cytometry; anti-proliferative activity; MTT assay; pharmaceutical industries; cytotoxicity; UV-visible spectroscopy; nanoparticle morphology; Centella asiatica leaf extract; biosynthesised nanoparticles; Fourier-transform infrared spectroscopy; GOLD NANOPARTICLES; GREEN SYNTHESIS; TOXICITY; NANOMATERIALS; MECHANISM;
D O I
10.1049/iet-nbt.2018.5069
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The aim of this study was to green synthesised silver nanoparticles (AgNPs) using Centella asiatica leaf extract and investigate the cytotoxic and apoptosis-inducing effects of these nanoparticles in MCF-7 breast cancer cell line. The characteristics and morphology of the green synthesised AgNPs were evaluated using transmission electron microscopy, scanning electron microscopy, UV-visible spectroscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. The MTT assay was used to investigate the anti-proliferative activity of biosynthesised nanoparticles in MCF-7 cells. Apoptosis test was performed using flow cytometry and expression of caspase 3 and 9 genes. The spherical AgNPs with an average size of 19.17nm were synthesised. The results showed that biosynthesised AgNPs exhibited cytotoxicity, anti-cancer, apoptosis induction, and increased expression of genes encoding for caspases 3 and 9 in MCF-7 cancer cells in a concentration- and time-dependent manner. It seems that green synthesised AgNPs have potential uses for pharmaceutical industries.
引用
收藏
页码:994 / 1002
页数:9
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