Specificity of PCR and Serological Assays in the Detection of Escherichia coli Shiga Toxin Subtypes

被引：67

作者：

Feng, Peter C. H. ^{[1
]}

Jinneman, Karen ^{[2
]}

Scheutz, Flemming ^{[3
]}

Monday, Steven R. ^{[1
]}

机构：

[1] US FDA, Div Microbiol, College Pk, MD 20740 USA

[2] US FDA, Pacific Reg Lab NW, Bothell, WA USA

[3] WHO Collaborating Ctr Reference & Res Escherichia, Copenhagen, Denmark

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 18期

关键词：

VARIANTS; STRAINS; IDENTIFICATION; ASSOCIATION; O157-H7; STEC; GENE; FOOD;

D O I：

10.1128/AEM.00370-11

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Specificity analysis for stx or Stx subtypes in Escherichia coli showed that the PCR assays we tested did not detect stx(1d) and stx(2f), and some also missed stx(2b) and stx(2g). Most of the serological assays examined did not detect Stx2c, Stx2e, Stx2f, and Stx2g, and some strain-to-strain variation in reactivity was observed for Stx2b.

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收藏

页码：6699 / 6702

页数：4

相关论文

共 23 条

[1] Evaluation of the anti-terminator Q933 gene as a marker for Escherichia coli O157:H7 with high Shiga toxin production [J].

Ahmad, Aqeel ;

Zurek, Ludek .

CURRENT MICROBIOLOGY, 2006, 53 (04) :324-328

[2] Comparative evaluation of the Ridascreen® Verotoxin enzyme immunoassay for detection of Shiga-toxin producing strains of Escherichia coli (STEC) from food and other sources [J].

Beutin, L. ;

Steinrueck, H. ;

Krause, G. ;

Steege, K. ;

Haby, S. ;

Hultsch, G. ;

Appel, B. .

JOURNAL OF APPLIED MICROBIOLOGY, 2007, 102 (03) :630-639

[3] Evaluation of the VTEC-screen "Seiken" test for detection of different types of Shiga toxin (verotoxin)-producing Escherichia coli (STEC) in human stool samples [J].

Beutin, L ;

Zimmermann, S ;

Gleier, K .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2002, 42 (01) :1-8

[4] Rapid detection and isolation of shiga-like toxin (verocytotoxin)-producing Escherichia coli by direct testing of individual enterohemolytic colonies from washed sheep blood agar plates in the VTEC-RPLA assay [J].

Beutin, L ;

Zimmerman, S ;

Gleier, K .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (11) :2812-2814

[5] Evaluation of major types of Shiga toxin 2e-producing Escherichia coli bacteria present in food, pigs, and the environment as potential pathogens for humans [J].

Beutin, Lothar ;

Krueger, Ulrike ;

Krause, Gladys ;

Miko, Angelika ;

Martin, Annett ;

Strauch, Eckhard .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (15) :4806-4816

[6] Shiga toxin activatable by intestinal mucus in Escherichia coli isolated from humans:: Predictor for a severe clinical outcome [J].

Bielaszewska, Martina ;

Friedrich, Alexander W. ;

Aldick, Thomas ;

Schuerk-Bulgrin, Robin ;

Karch, Helge .

CLINICAL INFECTIOUS DISEASES, 2006, 43 (09) :1160-1167

[7] SIMULTANEOUS IDENTIFICATION OF STRAINS OF ESCHERICHIA-COLI SEROTYPE O157-H7 AND THEIR SHIGA-LIKE TOXIN TYPE BY MISMATCH AMPLIFICATION MUTATION ASSAY-MULTIPLEX PCR [J].

CEBULA, TA ;

PAYNE, WL ;

FENG, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (01) :248-250

[8] Multiplex PCR for detection of trait and virulence factors in enterohemorrhagic Escherichia coli serotypes [J].

Feng, P ;

Monday, SR .

MOLECULAR AND CELLULAR PROBES, 2000, 14 (06) :333-337

[9]

Feng P., 2002, Diarrheagenic Escherichia coli

[10] Virulence Characterization of Shiga-Toxigenic Escherichia coli Isolates from Wholesale Produce [J].

Feng, Peter C. H. ;

Councell, Terry ;

Keys, Christine ;

Monday, Steven R. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (01) :343-345