Pharmacological characterization and autoradiographic localization of dopamine receptors in the rat adrenal medulla

被引:4
作者
Barili, P
Zaccheo, D
Amenta, F
机构
[1] UNIV CAMERINO,IST FARMACOL,SEZ ANAT UMANA,I-62032 CAMERINO,ITALY
[2] UNIV GENOA,IST ANAT UMANA,GENOA,ITALY
关键词
dopamine receptor; adrenal medulla; radioligand binding; autoradiography; chromaffin cell; (rat);
D O I
10.1016/0014-2999(96)00386-X
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The pharmacological profile and the anatomical localization of dopamine D-1-like and D-2-like receptors were studied in sections of rat adrenal medulla, with radioligand binding and autoradiographic techniques, respectively. [H-3]([R]-(+)-chloro-2,3,4,5-tetrahydro-5-phenyl-l H-3benzazepin-al hemimaleate) (SCH 23390) was used as a ligand for dopamine D-1-like receptors and [H-3]spiperone was used as a ligand for dopamine D-2-like receptors. Radioligand binding and light microscope autoradiography did not show specific [3H]SCH 23390 binding in sections of rat adrenal medulla. This suggests that rat adrenal medulla does not express dopamine D-1-like receptors, [3H]Spiperone was specifically bound to sections of rat adrenal medulla. The binding was time-, temperature- and concentration-dependent, with a dissociation constant (K-d) of 1.05 nM and a maximum density of binding sites (B-max) of 100.2 +/- 3.8 fmol/mg tissue. The pharmacological profile of [H-3]spiperone binding to rat adrenal medulla was similar to that displayed by neostriatum, which is known to express dopamine D-2 receptors. Light microscope autoradiography showed the accumulation of specifically bound [3H]spiperone as silver grains within sections of adrenal medulla. Silver grains were found primarily over the cellular membrane of chromaffin cells. The above data indicate that chromaffin cells of the rat adrenal medulla express dopamine receptors belonging to the dopamine D-2 receptor subtype. These receptors are probably involved in the modulation of catecholamine release from chromaffin cells, as documented by functional studies.
引用
收藏
页码:129 / 135
页数:7
相关论文
共 26 条
[1]   CROSS-TALK BETWEEN M2 MUSCARINIC AND D1 DOPAMINE-RECEPTORS IN THE CAT ADRENAL-MEDULLA [J].
ALBILLOS, A ;
ABAD, F ;
GARCIA, AG .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 183 (03) :1019-1024
[2]  
AMENTA F, 1993, N-S ARCH PHARMACOL, V347, P147
[3]   DENSITY AND DISTRIBUTION OF DOPAMINE-RECEPTORS IN THE CARDIOVASCULAR-SYSTEM AND IN THE KIDNEY [J].
AMENTA, F .
JOURNAL OF AUTONOMIC PHARMACOLOGY, 1990, 10 :S11-S18
[4]  
AMENTA F, 1990, PERIPHERAL DOPAMINE, P39
[5]  
AMENTA PS, 1991, HISTOLOGY HUMAN MICR, P495
[6]   MULTIPLE FLUORESCENT LIGANDS FOR DOPAMINE-RECEPTORS .2. VISUALIZATION IN NEURAL TISSUES [J].
ARIANO, MA ;
KANG, HC ;
HAUGLAND, RP ;
SIBLEY, DR .
BRAIN RESEARCH, 1991, 547 (02) :208-222
[7]   ACTIVATION OF FACILITATION CALCIUM CHANNELS IN CHROMAFFIN CELLS BY D1 DOPAMINE-RECEPTORS THROUGH A CAMP PROTEIN KINASE-A-DEPENDENT MECHANISM [J].
ARTALEJO, CR ;
ARIANO, MA ;
PERLMAN, RL ;
FOX, AP .
NATURE, 1990, 348 (6298) :239-242
[8]  
BIGORNIA L, 1990, J PHARMACOL EXP THER, V252, P586
[9]   DOPAMINE-RECEPTORS ON ADRENAL CHROMAFFIN CELLS MODULATE CALCIUM-UPTAKE AND CATECHOLAMINE RELEASE [J].
BIGORNIA, L ;
SUOZZO, M ;
RYAN, KA ;
NAPP, D ;
SCHNEIDER, AS .
JOURNAL OF NEUROCHEMISTRY, 1988, 51 (04) :999-1006
[10]  
CHENG Y, 1973, BIOCHEM PHARMACOL, V22, P3099