Whole Cell Bioconversion of Ricinoleic Acid to 12-Ketooleic Acid by Recombinant Corynebacterium glutamicum-Based Biocatalyst

被引：16

作者：

Lee, Byeonghun ^{[1
]}

Lee, Saebom ^{[1
]}

Kim, Hyeonsoo ^{[1
]}

Jeong, Kijun ^{[2
]}

Park, Jinbyung ^{[3
]}

Park, Kyungmoon ^{[4
]}

Lee, Jinwon ^{[1
]}

机构：

[1] Sogang Univ, Dept Chem & Biomol Engn, Seoul 121742, South Korea

[2] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South Korea

[3] Ewha Womans Univ, Dept Food Sci & Engn, Seoul 120750, South Korea

[4] Hongik Univ, Dept Biol & Chem Engn, Sejong 339701, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2015年 / 25卷 / 04期

关键词：

12-Ketooleic acid; ricinoleic acid; secondary alcohol dehydrogenase; whole cell bioconversion; Corynebacterium glutamicum; OLEIC-ACID; ESCHERICHIA-COLI; FATTY-ACIDS; CONVERSION; EXPRESSION;

D O I：

10.4014/jmb.1501.01001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase of Micrococcus luteus NCTC2665 was studied. Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). The effects of pH, reaction temperature, and non-ionic detergent on recombinant C. glutamiucm whole cell bioconversion were examined. The determined optimal conditions for production of 12-ketooleic acid are pH 8.0, 35 degrees C, and 0.05 g/l Tween80. Under these conditions, recombinant C. glutamicum produces 3.3 mM 12-ketooleic acid, with a 72% (mol/mol) maximum conversion yield, and 1.1 g/l/h volumetric productivity in 2 h; and 3.9 mM 12-ketooleic acid, with a 74% (mol/mol) maximum conversion yield, and 0.69 g/l/h maximum volumetric productivity in 4 h of fermentation. This study constitutes the first report of significant production of 12-ketooleic acid using a recombinant Corynebacterium glutamicum-based biocatalyst.

引用

页码：452 / 458

页数：7

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