DNA extraction from Ascaris suum muscle tissue

被引：4

作者：

Di Mito, C ^{[1
]}

Betschart, B ^{[1
]}

机构：

[1] Univ Neuchatel, Inst Zool, CH-2007 Neuchatel, Switzerland

来源：

PARASITOLOGY RESEARCH | 1998年 / 84卷 / 07期

关键词：

CTAB; Polyphenol; Muscle Tissue; Extraction Procedure; Ascaris;

D O I：

10.1007/s004360050456

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A new method for the extraction of DNA from Ascaris suum muscle has been developed. It combines a standard SDS-based extraction with a plant DNA extraction procedure. The use of SDS and proteinase It allows the elimination of proteins, while CTAB and polyclar AT eliminate glycogen and polyphenols. The DNA thus obtained can easily be digested by endonucleases and amplified by PCR.

引用

页码：596 / 597

页数：2