A glycosaminoglycan microarray identifies the binding of SARS-CoV-2 spike protein to chondroitin sulfate E

被引：10
|
作者：
Watanabe, Tomoko [1 ]
Takeda, Ko [2 ]
Hiemori, Keiko [1 ]
Minamisawa, Toshikazu [2 ]
Tateno, Hiroaki [1 ]
机构：
[1] Natl Inst Adv Ind Sci & Technol, Cellular & Mol Biotechnol Res Inst, 1-1-1 Higashi, Tsukuba, Ibaraki 3058566, Japan
[2] Seikagaku Corp, Cent Res Lab, Higashiyamato, Japan
来源：
FEBS LETTERS | 2021年 / 595卷 / 18期
关键词：
chondroitin sulfate; glycosaminoglycan; heparan sulfate; microarray; S protein; SARS-CoV-2; GROWTH-FACTORS; INVASION; HEPARIN; CELLS;
D O I：
10.1002/1873-3468.14173
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Heparan sulfate (HS), a sulfated glycosaminoglycan (GAG), was reported to be a necessary host attachment factor that promotes SARS-CoV-2 infection. In this study, we developed GAG microarrays based on fluorescence detection for high-sensitivity screening of the GAG-binding specificity of proteins and applied it for the analysis of SARS-CoV-2 spike (S) protein. Among the 20 distinct GAGs, the S protein bound not only to heparin (HEP)/HS but also to chondroitin sulfate E (CSE) in a concentration-dependent manner. We then analyzed the specificity of each subunit of the S protein. While the S1 subunit showed exclusive binding to HEP, the S2 subunit also bound to CSE and HEP/HS. CSE might act as an alternative attachment factor for HS in SARS-CoV-2 infection.
引用
收藏
页码：2341 / 2349
页数：9
相关论文
共 50 条
  • [41] Effects of Different Types of Recombinant SARS-CoV-2 Spike Protein on Circulating Monocytes' Structure
    Vettori, Marco
    Dima, Francesco
    Henry, Brandon Michael
    Carpene, Giovanni
    Gelati, Matteo
    Celegon, Giovanni
    Salvagno, Gian Luca
    Lippi, Giuseppe
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (11)
  • [42] Mutation and codon bias analysis of the spike protein of Omicron, the recent variant of SARS-CoV-2
    Lu, Yunbiao
    Wang, Weixiu
    Liu, Hao
    Li, Yue
    Yan, Ge
    Franzo, Giovanni
    Dai, Jianjun
    He, Wan-Ting
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2023, 250
  • [43] Potential clinical drugs as covalent inhibitors of the priming proteases of the spike protein of SARS-CoV-2
    Li, Qizhang
    Wang, Zhiying
    Zheng, Qiang
    Liu, Sen
    COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, 2020, 18 : 2200 - 2208
  • [44] SARS-CoV-2 deregulates the vascular and immune functions of brain pericytes via Spike protein
    Khaddaj-Mallat, Rayan
    Aldib, Natija
    Bernard, Maxime
    Paquette, Anne-Sophie
    Ferreira, Aymeric
    Lecordier, Sarah
    Saghatelyan, Armen
    Flamand, Louis
    ElAli, Ayman
    NEUROBIOLOGY OF DISEASE, 2021, 161
  • [45] SARS-CoV-2 Proteins Microarray by Photoimmobilization for Serodiagnosis of the Antibodies
    Kashiwagi, Hiroharu
    Morishima, Nobuhiro
    Obuse, Sei
    Isoshima, Takashi
    Akimoto, Jun
    Ito, Yoshihiro
    BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 2021, 94 (10) : 2435 - 2443
  • [46] SARS-CoV-2 Spike Protein-Derived Cyclic Peptides as Modulators of Spike Interaction with GRP78
    Johnson, Nicholas
    Pattinson, Craig
    Burgoyne, Kate
    Hijazi, Karolin
    Houssen, Wael E.
    Milne, Bruce F.
    CHEMBIOCHEM, 2024, 25 (12)
  • [47] Endothelial glycocalyx shields the interaction of SARS-CoV-2 spike protein with ACE2 receptors
    Targosz-Korecka, Marta
    Kubisiak, Agata
    Kloska, Damian
    Kopacz, Aleksandra
    Grochot-Przeczek, Anna
    Szymonski, Marek
    SCIENTIFIC REPORTS, 2021, 11 (01)
  • [48] ACE2-Independent Interaction of SARS-CoV-2 Spike Protein with Human Epithelial Cells Is Inhibited by Unfractionated Heparin
    Partridge, Lynda J.
    Urwin, Lucy
    Nicklin, Martin J. H.
    James, David C.
    Green, Luke R.
    Monk, Peter N.
    CELLS, 2021, 10 (06)
  • [49] Structural and Immunoreactivity Properties of the SARS-CoV-2 Spike Protein upon the Development of an Inactivated Vaccine
    Kordyukova, Larisa V.
    Moiseenko, Andrey V.
    Serebryakova, Marina V.
    Shuklina, Marina A.
    Sergeeva, Maria V.
    Lioznov, Dmitry A.
    Shanko, Andrei V.
    VIRUSES-BASEL, 2023, 15 (02):
  • [50] Characterization of heparin and severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) spike glycoprotein binding interactions
    Kim, So Young
    Jin, Weihua
    Sood, Amika
    Montgomery, David W.
    Grant, Oliver C.
    Fuster, Mark M.
    Fu, Li
    Dordick, Jonathan S.
    Woods, Robert J.
    Zhang, Fuming
    Linhardt, Robert J.
    ANTIVIRAL RESEARCH, 2020, 181
12345