A glycosaminoglycan microarray identifies the binding of SARS-CoV-2 spike protein to chondroitin sulfate E

被引：10
|
作者：
Watanabe, Tomoko [1 ]
Takeda, Ko [2 ]
Hiemori, Keiko [1 ]
Minamisawa, Toshikazu [2 ]
Tateno, Hiroaki [1 ]
机构：
[1] Natl Inst Adv Ind Sci & Technol, Cellular & Mol Biotechnol Res Inst, 1-1-1 Higashi, Tsukuba, Ibaraki 3058566, Japan
[2] Seikagaku Corp, Cent Res Lab, Higashiyamato, Japan
来源：
FEBS LETTERS | 2021年 / 595卷 / 18期
关键词：
chondroitin sulfate; glycosaminoglycan; heparan sulfate; microarray; S protein; SARS-CoV-2; GROWTH-FACTORS; INVASION; HEPARIN; CELLS;
D O I：
10.1002/1873-3468.14173
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Heparan sulfate (HS), a sulfated glycosaminoglycan (GAG), was reported to be a necessary host attachment factor that promotes SARS-CoV-2 infection. In this study, we developed GAG microarrays based on fluorescence detection for high-sensitivity screening of the GAG-binding specificity of proteins and applied it for the analysis of SARS-CoV-2 spike (S) protein. Among the 20 distinct GAGs, the S protein bound not only to heparin (HEP)/HS but also to chondroitin sulfate E (CSE) in a concentration-dependent manner. We then analyzed the specificity of each subunit of the S protein. While the S1 subunit showed exclusive binding to HEP, the S2 subunit also bound to CSE and HEP/HS. CSE might act as an alternative attachment factor for HS in SARS-CoV-2 infection.
引用
收藏
页码：2341 / 2349
页数：9
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