Chemical composition, FTIR Studies and Antibacterial Activity of Passiflora edulis f. edulis (Fruit)

被引:8
|
作者
Rizwana, Humaira [1 ]
Al Otibi, Fatimah [1 ]
Al-malki, Nouf [2 ]
机构
[1] King Saud Univ, Dept Bot & Microbiol, Coll Sci, Riyadh, Saudi Arabia
[2] King Fahd Specialist Hosp Dammam, POB 15215, Dammam, Saudi Arabia
来源
JOURNAL OF PURE AND APPLIED MICROBIOLOGY | 2019年 / 13卷 / 04期
关键词
Passion fruit; pulp; Rind; scanning electron microscopy; Gas chromatography-mass spectrometry; Fourier transform infrared; ANTIFUNGAL ACTIVITY; MEDICINAL-PLANTS; EXTRACTS; L;
D O I
10.22207/JPAM.13.4.64
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Because of the strong interest in the use of bio-products as alternatives to chemically derived antibiotics or antimicrobial agents, passion fruits extracts were evaluated for their antibacterial activity and chemical composition. Various solvent extracts of P. edulis were screened for their antibacterial activity by Agar well diffusion technique against an array of pathogenic bacteria (Gram positive and negative). Macro dilution technique was used to determine the Minimum inhibitory concentration of the potent extracts. Bacterial strain showing significant inhibition was further subjected to scanning electron microcopy (SEM) and the morphological changes induced by extracts were noted. Chemical composition of extracts showing strong antibacterial activity was determined by GC-MS and FTIR analysis. Extracts of Passion fruit (pulp with seeds) show significant inhibitory effects against test isolates but in a variable manner. Amongst all the test isolates Bacillus subtilis showed maximum inhibition followed by E. coli and P. aeruginosa. Ethyl acetate extracts had the least activity against the tested microorganisms. Gas chromatography-mass spectrometry of ethanol extracts showed the presence of important chemicals, such as Tetracosamethyl-cyclododecasiloxane; Dodecanoic acid, 10-methyl-, methyl ester cyclosiloxane, hexadecamethyl; 3-isopropoxy-1,1,1,7,7,7-hexamethyl-3,5,5-tris (trimethylsiloxy)tetrasil; 9-hexadecenoic acid, 9-octadecenyl ester, (Z,Z)- Fourier transform infrared studies revealed important functional groups which included phenols, esters, flavonoids, aromatic compounds, and alcohols. Significant antibacterial activity of the extracts could be attributed to phenolic compounds, esters and other chemical components identified in ethanolic extracts. Scanning electron micrographs of B. subtilis treated with ethanol extracts showed distorted shapes, rough and corrugated cell margins, and aggregations of cells. Our data depict the significant antimicrobial activity of extracts against Gram positive bacteria while the Gram negative bacteria exhibited weak inhibition by all the extracts. Based on our findings, passion fruits can be used in preparations of antimicrobial formulations against Gram positive microorganisms especially B. subtilis.
引用
收藏
页码:2489 / 2498
页数:10
相关论文
共 50 条
  • [1] Flavonoids Accumulation in Fruit Peel and Expression Profiling of Related Genes in Purple (Passiflora edulis f. edulis) and Yellow (Passiflora edulis f. flavicarpa) Passion Fruits
    Shi, Meng
    Ali, Muhammad Moaaz
    He, Yinying
    Ma, Songfeng
    Rizwan, Hafiz Muhammad
    Yang, Qiang
    Li, Binqi
    Lin, Zhimin
    Chen, Faxing
    PLANTS-BASEL, 2021, 10 (11):
  • [2] A simple and fast Agrobacterium-mediated transformation system for passion fruit KPF4 (Passiflora edulis f. edulis × Passiflora edulis f. flavicarpa)
    Lydia K. Asande
    Richard O. Omwoyo
    Richard O. Oduor
    Evans N. Nyaboga
    Plant Methods, 16
  • [3] Initial development of passion fruit trees (Passiflora edulis f. flavicarpa, P. edulis f. edulis and P. alata) grafted onto Passiflora cincinnata
    Zucareli, Valdir
    Ono, Elizabeth Orika
    Fernandes Boaro, Carmen Silvia
    Brambilla, Wilian Polaco
    SEMINA-CIENCIAS AGRARIAS, 2014, 35 (05): : 2325 - 2339
  • [4] Chemical composition of essential oils of Passiflora edulis f. flavicarpa agroindustrial waste
    Choez-Guaranda, Ivan
    Ortega, Arnold
    Miranda, Migdalia
    Manzano, Patricia
    EMIRATES JOURNAL OF FOOD AND AGRICULTURE, 2017, 29 (06): : 458 - 462
  • [5] BIOTECHNOLOGICAL POTENTIAL OF PURPLE PASSION FRUIT ENDOPHYTIC FUNGI (Passiflora edulis f. edulis; Passifloraceae)
    Hurtado-Clopatosky, Stephany
    Marina Melgarejo, Luz
    Cruz-Castiblanco, Ginna Natalia
    Hoyos-Carvajal, Lilliana Maria
    ACTA BIOLOGICA COLOMBIANA, 2023, 28 (03): : 449 - 459
  • [6] Viruses infecting purple passion fruit (Passiflora edulis f. edulis) in southwestern Antioquia, Colombia
    Cardona, Daniela
    Higuita, Monica
    Posada, Jaime
    Gallo, Yuliana
    Marin, Mauricio
    Gutierrez, Pablo
    ARCHIVES OF PHYTOPATHOLOGY AND PLANT PROTECTION, 2022, 55 (12) : 1394 - 1409
  • [7] Optimization of in vitro organogenesis in passion fruit (Passiflora edulis F. flavicarpa)
    Trevisan, F
    Mendes, BMJ
    SCIENTIA AGRICOLA, 2005, 62 (04): : 346 - 350
  • [8] Floral and reproductive biology of the "gulupa" Passiflora edulis Sims f. edulis
    Angel-Coca, Catalina
    Nates-Parra, Guiomar
    Ospina-Torres, Rodulfo
    Melo Ortiz, Carlos Daniel
    Amaya-Marquez, Marisol
    CALDASIA, 2011, 33 (02) : 433 - 451
  • [9] Yellow Passion Fruit Seed Oil (Passiflora edulis f. flavicarpa): Physical and Chemical Characteristics
    Malacrida, Cassia Roberta
    Jorge, Neuza
    BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, 2012, 55 (01) : 127 - 134
  • [10] Chemical composition of purple passion fruit (Passiflora edulis var. Sims edulis) seed oil
    Giuffre, A. M.
    RIVISTA ITALIANA DELLE SOSTANZE GRASSE, 2007, 84 (02): : 87 - 93