Antioxidant and antigenotoxic activities in Acacia salicina extracts and its protective role against DNA strand scission induced by hydroxyl radical

被引：19
作者：
Chatti, Ines Bouhlel [1 ]
Boubaker, Jihed [1 ]
Skandrani, Ines [1 ]
Bhouri, Wissem [1 ]
Ghedira, Kamel [1 ]
Ghedira, Leila Chekir [1 ]
机构：
[1] Fac Pharm Med Dent Monastir, Unite Pharmacognosie Biol Mol UR 07 03 99, Monastir 5000, Tunisia
来源：
FOOD AND CHEMICAL TOXICOLOGY | 2011年 / 49卷 / 08期
关键词：
A; salicina; ABTS.(+) assay; NBT assay; Plasmid DNA damage; Ames assay; IN-VITRO; LIPID-PEROXIDATION; COMPONENTS; NILOTICA; DISEASES;
D O I：
10.1016/j.fct.2011.04.022
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The antioxidant potency of Acacia salicina extracts was investigated. Total antioxidant capacity was determined using an ABTS.(+). assay. Superoxide radical scavenging was measured using riboflavin-light-nitro blue tetrazolium (NBT) assay. In addition, the content of phenols, total flavonoids and sterols were measured in the tested extracts. The petroleum ether exhibited a potent scavenging activity toward ABTS radical cations. Whereas, chloroform extract showed the highest activity against superoxides radicals and was also able to protect pKS plasmid DNA against hydroxyl radicals induced DNA damages. The antimutagenicity of these extracts was assayed using the Ames assay against Salmonella typhimurium TA98 and S. typhimurium TA 1535 tester strains at different concentrations. These extracts decreased significantly the mutagenecity induced by sodium azide (SA) and 4-nitro-o-phenylenediamine (NOP). The antioxidant and antimutagenecity activities exhibited by A. salicina depended on the chemical composition of the tested extracts. (C) 2011 Elsevier Ltd. All rights reserved.
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收藏
页码：1753 / 1758
页数：6
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