Analysis of blood processing conditions to obtain high-quality total RNA from human leukocyte concentrate

被引：0

作者：

Perez, I. A. G. ^{[1
]}

Santana, S. P. ^{[1
]}

Argudin, T. D. ^{[1
]}

Gardon, D. O. P. ^{[1
]}

机构：

[1] Ctr Genet Engn & Biotechnol, Genomics & Diagnost Div, Havana, Cuba

来源：

GENETICS AND MOLECULAR RESEARCH | 2007年 / 6卷 / 02期

关键词：

leukocyte; RNA; RT-PCR; Adrenomedullin;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Blood samples are used as a biological source to discover biomarkers of hematological and non-hematological disorders. The present study shows the impact of different experimental conditions associated with cell lysis buffer, TRI-reagent protocol and blood cell storage buffer and their correlation with the quantity, quality and Adrenomedullin gene expression levels of total RNA when RT-PCR technique is used. A leukocyte cell bank protocol is also proposed for further mRNA expression analysis using RNAlater as storage buffer. There is evidence that total RNA isolated from leukocyte concentrate stored for 1 month at -70 degrees C did not show significant differences concerning quality, purity and Adrenomedullin gene expression compared with the freshly processed leukocyte sample.

引用

页码：298 / 307

页数：10

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