Toxic Effect of Zinc Ions Is Accompanied by Acidification of the Cytoplasm in Cultured Rat Cerebellar Granule Neurons

被引：1

作者：

Shedenkova, M. O. ^{[1
,2
]}

Stelmashook, E. V. ^{[1
]}

Isaev, N. K. ^{[1
,2
]}

机构：

[1] Res Ctr Neurol, Moscow, Russia

[2] M V Lomonosov Moscow State Univ, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2022年 / 173卷 / 04期

关键词：

survival; neurons; zinc ions; acidosis; pH; ACIDOSIS;

D O I：

10.1007/s10517-022-05578-0

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Addition of 0.07 mM zinc ions to the culture medium induced death of cerebellar granule neurons. This was preceded by a decrease in intracellular pH by 0.86 +/- 0.12 and an increase in the level of intracellular zinc registered by increasing fluorescence of FluoZin3. However, intracellular acidosis caused by acidification of the culture medium to pH 6.0 was not toxic to neurons, and even significantly increased their survival under the action of zinc ions. In general, the overload of the neurons cytoplasm with zinc ions causes acidification of the cytoplasm, which is probably associated with the activation of Zn2(+)/H+ exchangers and is a protective mechanism for the neurocytotoxic effect of zinc ions.

引用

页码：539 / 543

页数：5

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