Detection of Circulating Tumor Cells and Circulating Tumor Microemboli in Gastric Cancer

被引：57

作者：

Zheng, Xiumei ^{[1
]}

Fan, Li ^{[1
]}

Zhou, Pengfei ^{[2
]}

Ma, Hong ^{[1
]}

Huang, Shaoyi ^{[2
]}

Yu, Dandan ^{[1
]}

Zhao, Lei ^{[1
]}

Yang, Shengli ^{[1
]}

Liu, Jun ^{[1
]}

Huang, Ai ^{[1
]}

Cai, Congli ^{[2
]}

Dai, Xiaomeng ^{[1
]}

Zhang, Tao ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Canc Ctr, Union Hosp, 1277 Jiefang Ave, Wuhan 430022, Hubei, Peoples R China

[2] Wuhan YZY Med Sci & Technol Co Ltd, 666 Gaoxin Rd, Wuhan, Hubei, Peoples R China

来源：

TRANSLATIONAL ONCOLOGY | 2017年 / 10卷 / 03期

基金：

中国国家自然科学基金;

关键词：

PROGNOSTIC-SIGNIFICANCE; CLINICAL-SIGNIFICANCE; PERIPHERAL-BLOOD; BREAST-CANCER; SURVIVAL; SIZE; METASTASIS; CA125; PROGRESSION; EXPRESSION;

D O I：

10.1016/j.tranon.2017.02.007

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

PURPOSE: Gastric cancer studies indicated a potential correlation between circulating tumor cells (CTCs) in peripheral blood and tumor relapse/metastasis. The prevalence and significance of circulating tumor microemboli (CTM) in gastric cancer remain unknown. We investigated the prevalence and prognostic value of CTCs and CTM for progression-free survival (PFS) and overall survival (OS) in gastric cancer patients. METHODS: Eighty-one gastric cancer patients consented to provide 5 ml of peripheral blood before systematic therapy. CTCs and CTM were isolated using isolation by size of epithelial tumor cells and characterized by cytopathologists. For 41 stage IV gastric cancer patients, CTM was investigated as a potential biomarker to predict prognosis. RESULTS: CTCs were detected in 51 patients; the average count was 1.81. In clinical stage I, II, III, and IV patients, the average CTC counts were 1.40, 0.67, 1.24, and 2.71, respectively. CTM were detected in 3 of 33 clinical stage I to IIIb patients, at an average of 0.12 (0-2). CTM were detected in 13 of 53 clinical stage IIIc to IV patients, at an average of 1.26 (0-22). In stage IV patients, CTM positivity correlated with the CA125 level. PFS and OS in CTM-positive patients were significantly lower than in CTM-negative patients (P < .001). CTM positivity was an independent factor for determining the PFS (P = .016) and OS (P = .003) of stage IV patients in multivariate analysis. Using markers of the epithelial-mesenchymal transition, single CTCs were divided into three phenotypes including epithelial CTCs, biphenotypic epithelial/mesenchymal CTCs, and mesenchymal CTCs. For CTM, CK-/Vimentin+/CD45- and CK+/Vimentin+/CD45- phenotypes were observed, but the CK+/Vimentin-/CD45- CTM phenotype was not. CA125 was detected in gastric cancer cell lines BGC823 and MGC803. CONCLUSIONS: In stage IV patients, CTM positivity was correlated with serum CA125 level. CTM were an independent predictor of shorter PFS and OS in stage IV patients. Thus, CTM detection may be a useful tool to predict prognosis in stage IV patients.

引用

页码：431 / 441

页数：11

共 52 条

[1] Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis [J].

Aceto, Nicola ;

Bardia, Aditya ;

Miyamoto, David T. ;

Donaldson, Maria C. ;

Wittner, Ben S. ;

Spencer, Joel A. ;

Yu, Min ;

Pely, Adam ;

Engstrom, Amanda ;

Zhu, Huili ;

Brannigan, Brian W. ;

Kapur, Ravi ;

Stott, Shannon L. ;

Shioda, Toshi ;

Ramaswamy, Sridhar ;

Ting, David T. ;

Lin, Charles P. ;

Toner, Mehmet ;

Haber, Daniel A. ;

Maheswaran, Shyamala .

CELL, 2014, 158 (05) :1110-1122

[2] Mesothelin expression correlates with prolonged patient survival in gastric cancer [J].

Baba, Kenji ;

Ishigami, Sumiya ;

Arigami, Takaaki ;

Uenosono, Yoshikazu ;

Okumura, Hiroshi ;

Matsumoto, Masataka ;

Kurahara, Hiroshi ;

Uchikado, Yuto ;

Kita, Yoshiaki ;

Kijima, Yuko ;

Kitazono, Masaki ;

Shinchi, Hiroyuki ;

Ueno, Shinichi ;

Natsugoe, Shoji .

JOURNAL OF SURGICAL ONCOLOGY, 2012, 105 (02) :195-199

[3] Notch-Jagged signalling can give rise to clusters of cells exhibiting a hybrid epithelial/mesenchymal phenotype [J].

Boareto, Marcelo ;

Jolly, Mohit Kumar ;

Goldman, Aaron ;

Pietila, Mika ;

Mani, Sendurai A. ;

Sengupta, Shiladitya ;

Ben-Jacob, Eshel ;

Levine, Herbert ;

Onuchic, Jose N. .

JOURNAL OF THE ROYAL SOCIETY INTERFACE, 2016, 13 (118)

[4] A Perspective on Cancer Cell Metastasis [J].

Chaffer, Christine L. ;

Weinberg, Robert A. .

SCIENCE, 2011, 331 (6024) :1559-1564

[5] Relationship of circulating tumor cells to tumor response, progression-free survival, and overall survival in patients with metastatic colorectal cancer [J].

Cohen, Steven J. ;

Punt, Cornelis J. A. ;

Iannotti, Nicholas ;

Saidman, Bruce H. ;

Sabbath, Kert D. ;

Gabrail, Nashat Y. ;

Picus, Joel ;

Morse, Michael ;

Mitchell, Edith ;

Miller, M. Craig ;

Doyle, Gerald V. ;

Tissing, Henk ;

Terstappen, Leon W. M. M. ;

Meropol, Neal J. .

JOURNAL OF CLINICAL ONCOLOGY, 2008, 26 (19) :3213-3221

[6] Circulating tumor cells, disease progression, and survival in metastatic breast cancer [J].

Cristofanilli, M ;

Budd, GT ;

Ellis, MJ ;

Stopeck, A ;

Matera, J ;

Miller, MC ;

Reuben, JM ;

Doyle, GV ;

Allard, WJ ;

Terstappen, LWMM ;

Hayes, DF .

NEW ENGLAND JOURNAL OF MEDICINE, 2004, 351 (08) :781-791

[7] Circulating Tumor Cells Predict Survival Benefit from Treatment in Metastatic Castration-Resistant Prostate Cancer [J].

de Bono, Johann S. ;

Scher, Howard I. ;

Montgomery, R. Bruce ;

Parker, Christopher ;

Miller, M. Craig ;

Tissing, Henk ;

Doyle, Gerald V. ;

Terstappen, Leon W. W. M. ;

Pienta, Kenneth J. ;

Raghavan, Derek .

CLINICAL CANCER RESEARCH, 2008, 14 (19) :6302-6309

[8] Application of a Filtration- and Isolation-by-Size Technique for the Detection of Circulating Tumor Cells in Cutaneous Melanoma [J].

De Giorgi, Vincenzo ;

Pinzani, Pamela ;

Salvianti, Francesca ;

Panelos, John ;

Paglierani, Milena ;

Janowska, Agata ;

Grazzini, Marta ;

Wechsler, Janine ;

Orlando, Claudio ;

Santucci, Marco ;

Lotti, Torello ;

Pazzagli, Mario ;

Massi, Daniela .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2010, 130 (10) :2440-2447

[9] The presence of clustered circulating tumor cells (CTCs) and circulating cytokines define an aggressive phenotype in metastatic colorectal cancer [J].

Divella, Rosa ;

Daniele, Antonella ;

Abbate, Ines ;

Bellizzi, Antonia ;

Savino, Eufemia ;

Simone, Giovanni ;

Giannone, Grazia ;

Giuliani, Francesco ;

Fazio, Vito ;

Gadaleta-Caldarola, Gennaro ;

Gadaleta, Cosimo Damiano ;

Lolli, Ivan ;

Sabba, Carlo ;

Mazzocca, Antonio .

CANCER CAUSES & CONTROL, 2014, 25 (11) :1531-1541

[10] A direct comparison of CellSearch and ISET for circulating tumour-cell detection in patients with metastatic carcinomas [J].

Farace, F. ;

Massard, C. ;

Vimond, N. ;

Drusch, F. ;

Jacques, N. ;

Billiot, F. ;

Laplanche, A. ;

Chauchereau, A. ;

Lacroix, L. ;

Planchard, D. ;

Le Moulec, S. ;

Andre, F. ;

Fizazi, K. ;

Soria, J. C. ;

Vielh, P. .

BRITISH JOURNAL OF CANCER, 2011, 105 (06) :847-853

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