Improvement of a method for chain-length distribution analysis of wheat amylopectin

被引：59
|
作者：
Nagamine, T
Komae, K
机构：
[1] Crop Breeding Department, National Agriculture Research Center, Tsukuba, Ibaraki 305
来源：
JOURNAL OF CHROMATOGRAPHY A | 1996年 / 732卷 / 02期
关键词：
chain length distribution; amylopectin; starch; maltooligosaccharides;
D O I：
10.1016/0021-9673(95)01229-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
For the chain-length distribution analysis of wheat amylopectin using high-performance anion-exchange chromatography (HPAEC) under alkaline conditions, the reduction of amylopectin isoamylolyzate with sodium borohydride facilitated obtaining reproducible chromatograms without both alkaline degradation and epimerization of maltooligosaccharides in aqueous NaOH solution. In a comparison of the chromatograms of isoamylolyzates of wheat starch and its fractionated amylopectin, reproducible results were also obtained. The improved method presented here, based on the use of starch isoamylolyzates reduced with sodium borohydride, is available for performing automated analysis of the chain-length distribution of wheat amylopectin by HPAEC equipped with an autosampler.
引用
收藏
页码：255 / 259
页数：5
相关论文
共 50 条
  • [1] GAS-CHROMATOGRAPHIC METHOD FOR ESTIMATION OF CHAIN-LENGTH OF AMYLOPECTIN
    YAMADA, T
    HISAMATSU, M
    TAKI, M
    JOURNAL OF THE AGRICULTURAL CHEMICAL SOCIETY OF JAPAN, 1975, 49 (03): : 163 - 167
  • [2] Effects of temperature during ripening on amylopectin chain-length distribution of 'Koshihikari' and 'Ichihomare'
    Kobayashi, Asako
    Machida, Yoshie
    Watanabe, Syuto
    Morozumi, Yusaku
    Nakaoka, Fumihiro
    Hayashi, Takeshi
    Tomita, Katsura
    PLANT PRODUCTION SCIENCE, 2022, 25 (02) : 250 - 259
  • [3] EFFECT OF AMYLOPECTIN EXTERNAL CHAIN-LENGTH ON THE KINETICS OF RETROGRADATION OF CONCENTRATED WHEAT-STARCH GELS
    CIACCO, CF
    FERNANDES, JLA
    ZOEGA, RH
    ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS, 1979, 51 (02): : 249 - 253
  • [4] RELATIONSHIP BETWEEN THE DISTRIBUTION OF THE CHAIN-LENGTH OF AMYLOPECTIN AND THE CRYSTALLINE-STRUCTURE OF STARCH GRANULES
    HIZUKURI, S
    CARBOHYDRATE RESEARCH, 1985, 141 (02) : 295 - 306
  • [5] Haplotype Analysis of Wx and Alk Genes and Amylopectin Chain-Length Distribution among Kazakhstan Glutinous Rice Lines
    Sartbayeva, Innabat
    Usenbekov, Bakdaulet
    Rysbekova, Aiman
    Kazkeyev, Dauren
    Zhanbyrbayev, Yeldos
    Batayeva, Dariga
    Berkimbay, Khorlan
    Zhambakin, Kabyl
    Matsuba, Shuichi
    Umemoto, Takayuki
    JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY, 2017, 51 (02): : 147 - 153
  • [6] AN HPLC METHOD FOR DETERMINING CHAIN-LENGTH DISTRIBUTION IN SOME GLYCOGENS
    CHEETHAM, NWH
    HANSAWEK, N
    SAECOU, P
    CARBOHYDRATE RESEARCH, 1991, 215 (01) : 59 - 65
  • [7] AN EFFICIENT METHOD FOR COMPUTING CHAIN-LENGTH DISTRIBUTIONS FOR CHAIN-LENGTH DEPENDENT REACTIVITY
    MAGALHAES, FD
    COSTA, MRN
    POLYMER REACTION ENGINEERING /, 1989, : 311 - 320
  • [8] Effects of amylose and amylopectin chain-length distribution on the kinetics of long-term rice starch retrogradation
    Li, Cheng
    Hu, Yiming
    Li, Enpeng
    FOOD HYDROCOLLOIDS, 2021, 111
  • [9] Origin of defects in assembled supramolecular structures of sweet potato starches with different amylopectin chain-length distribution
    Noda, Takahiro
    Isono, Naoto
    Krivandin, Alexey V.
    Shatalova, Olga V.
    Blaszczak, Wioletta
    Yuryev, Vladimir P.
    CARBOHYDRATE POLYMERS, 2009, 76 (03) : 400 - 409
  • [10] CHAIN LENGTH AND CHAIN-LENGTH DISTRIBUTION OF WOOD CELLULOSE AS COMPARED WITH COTTON
    HEUSER, E
    JORGENSEN, L
    TAPPI, 1951, 34 (02): : 57 - 67
12345