Toxicological effects of 3-methyl-4-nitrophenol on mouse ovarian and testicular cell proliferation, apoptosis and oocyte maturation

被引：12

作者：

Han, Yingying ^{[1
]}

Zhang, Shuhao ^{[1
]}

Wang, Ziyi ^{[1
]}

Zhang, Lin ^{[1
]}

Zhang, Fengwei ^{[2
]}

Sun, Fangzhou ^{[1
]}

Zhang, Haolin ^{[1
]}

Yuan, Zhengrong ^{[1
]}

Zhang, Cheng ^{[3
]}

Weng, Qiang ^{[1
]}

机构：

[1] Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China

[2] Univ Hong Kong, Sch Biol Sci, Hong Kong, Peoples R China

[3] Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China

来源：

REPRODUCTIVE TOXICOLOGY | 2018年 / 82卷

基金：

北京市自然科学基金; 中国国家自然科学基金;

关键词：

3-Methyl-4-nitrophenol; Apoptosis; Cell proliferation; Oocyte maturation; DIESEL EXHAUST PARTICLES; ANTI-ANDROGENIC ACTIVITY; FOLLICULAR DEVELOPMENT; INHIBITORS P27(KIP1); GRANULOSA-CELLS; CORPUS-LUTEUM; EXPRESSION; SURVIVAL; CYCLE; PROGESTERONE;

D O I：

10.1016/j.reprotox.2018.10.005

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of the present study was to reveal the effects of 3-methyl-4-nitrophenol (PNMC) on mouse gonadal cell proliferation and apoptosis. Immature female and male mice were intramuscularly injected with 100 mg/kg PNMC or vehicle every five days. One-month later, ovarian rather than testicular weights were significantly decreased. The positive terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling staining was enhanced in both testicular seminiferous epithelium and ovarian corpus luteum. Further study in ovaries showed that PNMC treatment increased the protein expressions of PCNA, p21 and p27, and decreased the expression of Cyclin D2. Whereas PNMC had no significantly influence on those protein expressions in testes. In addition, we demonstrated that 100 nM PNMC significantly suppressed mouse oocyte meiotic resumption and cumulus cell expansion in vitro. These findings suggest that the reproductive toxicities of PNMC involve affecting the processes of gonadal cell apoptosis and proliferation.

引用

页码：94 / 102

页数：9

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