Immunogold localization of photosynthetic enzymes in leaves of various C4 plants, with particular reference to pyruvate orthophosphate dikinase

cellular localization of photosynthetic enzymes was investigated by immunogold electron microscopy for leaves of nine C-4 grasses (three NADP-malic enzyme (NADP-ME) subtype species, three NAD-malic enzyme (NAD-ME) subtype species, and three phosphoenolpyruvate carboxykinase (PCK) subtype species), two C-4 sedges (NADP-ME subtype species) and two C-4 dicots tan NADP-ME and an NADP/NAD-ME subtype species). In leaves of all species, immunogold labelling was present for phosphoenolpyruvate carboxylase in the cytosol of the mesophyll cells (MC) and for ribulose-1,5-bisphosphate carboxylase/oxygenase in the chloroplasts of the bundle sheath cells (BSC), However, considerable specific variation was found in the intercellular patterns of labelling for pyruvate orthophosphate dikinase (PPDK), In the NADP-ME grasses, two NAD-ME grasses, and the dicots, significant labelling for PPDK was present in the both the BSC and the MC chloroplasts, In the other NAD-ME grass, the PCK grasses, and the sedges, labelling for PPDK was present almost exclusively in the chloroplasts of the MC, These patterns were observed in the leaves of both young seedlings and mature plants, these results indicate that the accumulation of PPDK in leaves of C-4 plants is not necessarily restricted to the MC, although the chloroplasts of the MC accumulate more than those of the BSC,