Studies on interaction of water-soluble bridged porphyrin with DNA

被引：0

作者：

Wang, Kai ^{[1
]}

Zhang, Zhi ^{[1
]}

Guo, Qian-Ni ^{[1
]}

Bao, Xiao-Ping ^{[1
]}

Li, Zao-Ying ^{[1
]}

机构：

[1] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China

来源：

ACTA CHIMICA SINICA | 2007年 / 65卷 / 22期

关键词：

porphyrin dimer; DNA; apparent affinity binding constant; photocleavage;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A water-soluble porphyrin dimer (PD) containing eight positive charges, bridged by 4,4'-dicarboxy-2,2'-bipytidyl, has been synthesized. Taking meso-tetrakis(N-methylpyridinium-4-yl)porphyrin (H2TMPyP) as a reference compound, interaction of the water-soluble porphyrin dimer with DNA was investigated by using absorption, fluorescence, CD spectroscopy, and the apparent affinity binding constant (K-app) of PD binding to CT DNA was measured to be 1.2X10(6) L center dot mol(-1) by a competition method with ethidium bromide (EB) (that of H2TMPyP was 6.9X10(6) L center dot mol(-1)). The cleavage ability of PD to pBR322 plasmid DNA was studied by gel electrophoresis. The results suggest that the binding modes of PD and CT DNA involve in both of intercalation and outside binding.

引用

页码：2597 / 2603

页数：7

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