Long-term proliferation in culture and germline transmission of mouse male germline stem cells

被引：787

作者：

Kanatsu-Shinohara, M ^{[1
]}

Ogonuki, N

Inoue, K

Miki, H

Ogura, A

Toyokuni, S

Shinohara, T

机构：

[1] Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Sakyo Ku, Kyoto 6068501, Japan

[2] Kyoto Univ, Grad Sch Med, Dept Pathol & Biol Dis, Kyoto 6068501, Japan

[3] RIKEN, Inst Phys & Chem Res, Bioresouce Ctr, Tsukuba, Ibaraki 3050074, Japan

来源：

BIOLOGY OF REPRODUCTION | 2003年 / 69卷 / 02期

关键词：

developmental biology; gametogenesis; Sertoli cells; spermatogenesis; testis;

D O I：

10.1095/biolreprod.103.017012

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Spermatogenesis is a complex process that originates in a small population of spermatogonial stem cells. Here we report the in vitro culture of spermatogonial stem cells that proliferate for long periods of time. In the presence of glial cell line-derived neurotrophic factor, epidermal growth factor, basic fibroblast growth factor, and leukemia inhibitory factor, gonocytes isolated from neonatal mouse testis proliferated over a 5-month period (>10(14)-fold)and restored fertility to congenitally infertile recipient mice following transplantation into seminiferous tubules. Long-term spermatogonial stem cell culture will be useful for studying spermatogenesis mechanism and has important implications for developing new technology in transgenesis or medicine.

引用

页码：612 / 616

页数：5

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