UVA-induced oxidative damage in retinal pigment epithelial cells after H2O2 or sparfloxacin exposure

被引：11
|
作者：
Verna, LK [1 ]
Holman, SA [1 ]
Lee, VC [1 ]
Hoh, J [1 ]
机构：
[1] Univ Calif Riverside, Div Biomed Sci, Riverside, CA 92521 USA
来源：
CELL BIOLOGY AND TOXICOLOGY | 2000年 / 16卷 / 05期
关键词：
8-oxo-dG; 7,8-dihydro-8-oxo-2 '-deoxyguanosine; DNA damage; macular degeneration; retinal pigment epithelial cells;
D O I：
10.1023/A:1026798314217
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Retinal impairment is one of the leading causes of visual loss in an aging human population. To explore a possible cause for retinal damage in the human population, we have monitored DNA oxidation in human retinal pigment epithelial (RPE) cells after exposure to hydrogen peroxide (H2O2) or the quinolone antibacterial sparfloxacin. When H2O2- or sparfloxacin-exposed cells were further exposed to ultraviolet A (UVA) irradiation, oxidative damage to the DNA of these cells was greatly increased over baseline values. This RPE+pharmaceutical-UVA cell system was developed to mimic in vivo retinal degeneration, seen in mouse studies using quinolone and UVA exposure. DNA damage produced by sparfloxacin and UVA in RPE cells could be remedied by the use of antioxidants, indicating a possible in vivo method for prevention or minimization of retinal damage in humans
引用
收藏
页码：303 / 312
页数：10
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