Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals

被引：152

作者：

Magri, Giuliana ^{[1
]}

Comerma, Laura ^{[1
]}

Pybus, Marc ^{[1
]}

Sintes, Jordi ^{[1
]}

Llige, David ^{[1
]}

Segura-Garzon, Daniel ^{[1
]}

Bascones, Sabrina ^{[1
]}

Yeste, Ada ^{[1
]}

Grasset, Emilie K. ^{[2
,3
]}

Gutzeit, Cindy ^{[2
]}

Uzzan, Mathieu ^{[2
]}

Ramanujam, Meera ^{[4
]}

van Zelm, Menno C. ^{[5
,6
]}

Albero-Gonzalez, Raquel ^{[7
]}

Vazquez, Ivonne ^{[7
]}

Iglesias, Mar ^{[7
,8
]}

Serrano, Sergi ^{[7
,8
]}

Marquez, Lucia ^{[9
]}

Mercade, Elena ^{[10
]}

Mehandru, Saurabh ^{[2
]}

Cerutti, Andrea ^{[1
,2
,11
]}

机构：

[1] Inst Hosp Mar Invest Med IMIM, Program Inflammatory & Cardiovasc Disorders, Barcelona 08003, Spain

[2] Icahn Sch Med Mt Sinai, Inst Immunol, Dept Med, New York, NY 10029 USA

[3] Karolinska Inst, Karolinska Univ Hosp, Ctr Mol Med, Dept Med, S-17176 Stockholm, Sweden

[4] Boehringer Ingelheim Pharmaceut, Immunol & Resp Dis Res, Ridgefield, CT 06877 USA

[5] Monash Univ, Dept Immunol & Pathol, Melbourne, Vic 3004, Australia

[6] Alfred Hosp, Melbourne, Vic 3004, Australia

[7] Hosp Mar, Dept Pathol, Barcelona 08003, Spain

[8] Univ Autonoma Barcelona, Barcelona 08003, Spain

[9] Hosp Mar, Dept Gastroenterol, Barcelona 08003, Spain

[10] Univ Barcelona, Dept Biol Hlth & Environm, E-08028 Barcelona, Spain

[11] Catalan Inst Res & Adv Studies ICREA, Barcelona 08003, Spain

来源：

IMMUNITY | 2017年 / 47卷 / 01期

关键词：

LYMPHOID-TISSUE; SEQUENCING DATA; MICROBIOTA; GENERATION; RESPONSES; DIVERSIFICATION; REPERTOIRES; POPULATION; EXPRESSION; CAPACITIES;

D O I：

10.1016/j.immuni.2017.06.013

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Secretory immunoglobulin A (SIgA) enhances host-microbiota symbiosis, whereas SIgM remains poorly understood. We found that gut IgM(+) plasma cells (PCs) were more abundant in humans than mice and clonally related to a large repertoire of memory IgM(+) B cells disseminated throughout the intestine but rare in systemic lymphoid organs. In addition to sharing a gut-specific gene signature with memory IgA(+) B cells, memory IgM(+) B cells were related to some IgA(+) clonotypes and switched to IgA in response to T cell-independent or T cell-dependent signals. These signals induced abundant IgM which, together with SIgM from clonally affiliated PCs, recognized mucus-embedded commensals. Bacteria recognized by human SIgM were dually coated by SIgA and showed increased richness and diversity compared to IgA-only-coated or uncoated bacteria. Thus, SIgM may emerge from pre-existing memory rather than newly activated naive IgM(+) B cells and could help SIgA to anchor highly diverse commensal communities to mucus.

引用

页码：118 / +

页数：25

共 47 条

[1] Pathogenesis and treatment of gastrointestinal disease in antibody deficiency syndromes [J].

Agarwal, Shradha ;

Mayer, Lloyd .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2009, 124 (04) :658-664

[2] Human B-cell memory is shaped by age- and tissue-specific T-independent and GC-dependent events [J].

Aranburu, Alaitz ;

Mortari, Eva Piano ;

Baban, Anwar ;

Giorda, Ezio ;

Cascioli, Simona ;

Marcellini, Valentina ;

Scarsella, Marco ;

Ceccarelli, Sara ;

Corbelli, Sandro ;

Cantarutti, Nicoletta ;

De Vito, Rita ;

Inserra, Alessandro ;

Nicolosi, Luciana ;

Lanfranchi, Arnalda ;

Porta, Fulvio ;

Cancrini, Caterina ;

Finocchi, Andrea ;

Carsetti, Rita .

EUROPEAN JOURNAL OF IMMUNOLOGY, 2017, 47 (02) :327-344

[3] Metabolites produced by commensal bacteria promote peripheral regulatory T-cell generation [J].

Arpaia, Nicholas ;

Campbell, Clarissa ;

Fan, Xiying ;

Dikiy, Stanislav ;

van der Veeken, Joris ;

deRoos, Paul ;

Liu, Hui ;

Cross, Justin R. ;

Pfeffer, Klaus ;

Coffer, Paul J. ;

Rudensky, Alexander Y. .

NATURE, 2013, 504 (7480) :451-+

[4] IgTree©:: Creating immunoglobulin variable region gene lineage trees [J].

Barak, Michal ;

Zuckerman, Neta S. ;

Edelman, Hanna ;

Unger, Ron ;

Mehr, Ramit .

JOURNAL OF IMMUNOLOGICAL METHODS, 2008, 338 (1-2) :67-74

[5] Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization [J].

Bemark, Mats ;

Hazanov, Helena ;

Stromberg, Anneli ;

Komban, Rathan ;

Holmqvist, Joel ;

Koster, Sofia ;

Mattsson, Johan ;

Sikora, Per ;

Mehr, Ramit ;

Lycke, Nils Y. .

NATURE COMMUNICATIONS, 2016, 7

[6] The majority of intestinal IgA+ and IgG+ plasmablasts in the human gut are antigen-specific [J].

Benckert, Julia ;

Schmolka, Nina ;

Kreschel, Cornelia ;

Zoller, Markus Josef ;

Sturm, Andreas ;

Wiedenmann, Bertram ;

Wardemann, Hedda .

JOURNAL OF CLINICAL INVESTIGATION, 2011, 121 (05) :1946-1955

[7]

Biddle A., 2013, DIVERSITY-BASEL, V5, P627, DOI DOI 10.3390/d5030627

[8] QIIME allows analysis of high-throughput community sequencing data [J].

Caporaso, J. Gregory ;

Kuczynski, Justin ;

Stombaugh, Jesse ;

Bittinger, Kyle ;

Bushman, Frederic D. ;

Costello, Elizabeth K. ;

Fierer, Noah ;

Pena, Antonio Gonzalez ;

Goodrich, Julia K. ;

Gordon, Jeffrey I. ;

Huttley, Gavin A. ;

Kelley, Scott T. ;

Knights, Dan ;

Koenig, Jeremy E. ;

Ley, Ruth E. ;

Lozupone, Catherine A. ;

McDonald, Daniel ;

Muegge, Brian D. ;

Pirrung, Meg ;

Reeder, Jens ;

Sevinsky, Joel R. ;

Tumbaugh, Peter J. ;

Walters, William A. ;

Widmann, Jeremy ;

Yatsunenko, Tanya ;

Zaneveld, Jesse ;

Knight, Rob .

NATURE METHODS, 2010, 7 (05) :335-336

[9] Antibiotics in early life alter the murine colonic microbiome and adiposity [J].

Cho, Ilseung ;

Yamanishi, Shingo ;

Cox, Laura ;

Methe, Barbara A. ;

Zavadil, Jiri ;

Li, Kelvin ;

Gao, Zhan ;

Mahana, Douglas ;

Raju, Kartik ;

Teitler, Isabel ;

Li, Huilin ;

Alekseyenko, Alexander V. ;

Blaser, Martin J. .

NATURE, 2012, 488 (7413) :621-+

[10] Identification of a human splenic marginal zone B cell precursor with NOTCH2-dependent differentiation properties [J].

Descatoire, Marc ;

Weller, Sandra ;

Irtan, Sabine ;

Sarnacki, Sabine ;

Feuillard, Jean ;

Storck, Sebastien ;

Guiochon-Mantel, Anne ;

Bouligand, Jerome ;

Morali, Alain ;

Cohen, Joseph ;

Jacquemin, Emmanuel ;

Iascone, Maria ;

Bole-Feysot, Christine ;

Cagnard, Nicolas ;

Weill, Jean-Claude ;

Reynaud, Claude-Agnes .

JOURNAL OF EXPERIMENTAL MEDICINE, 2014, 211 (05) :986-1000

← 1 2 3 4 5 →