The evaluation of fast purification methods for preparing polymerase chain reaction (PCR) products for capillary electrophoresis analysis

被引：0

作者：

Devaney, JM ^{[1
]}

Marino, MA ^{[1
]}

Williams, PE ^{[1
]}

Weaver, KR ^{[1
]}

DelRio, SA ^{[1
]}

Turner, KA ^{[1
]}

Belgrader, P ^{[1
]}

机构：

[1] ARMED FORCES INST PATHOL,ARMED FORCES DNA IDENTIFICAT LAB,ROCKVILLE,MD 20850

来源：

APPLIED AND THEORETICAL ELECTROPHORESIS | 1996年 / 6卷 / 01期

关键词：

capillary electrophoresis; PCR; purification; salt;

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The analysis of crude polymerase chain reaction (PCR) products by capillary electrophoresis (CE) is often compromised by the presence of a high concentration of salt. Salt interferes with the electrokinetic injection and induces localized heating within the column, hence, PCR products must be desalted or cleaned-up prior to CE analysis. A variety of commercial clean-up systems are available that have been traditionally used to prepare PCR products for cloning, sequencing, and digestion with restriction enzymes. These systems were tested for their effectiveness in preparing PCR products for CE analysis and were evaluated based on CE resolution, salt removal, DNA recovery, processing time, and cost.

引用

页码：11 / 14

页数：4

共 17 条

[1] LIGATION-INDEPENDENT CLONING OF PCR PRODUCTS (LIC-PCR) [J].

ASLANIDIS, C ;

DEJONG, PJ .

NUCLEIC ACIDS RESEARCH, 1990, 18 (20) :6069-6074

[2] DNA-SEQUENCING BY CAPILLARY ELECTROPHORESIS - USE OF A 2-LASER 2-WINDOW INTENSIFIED DIODE-ARRAY DETECTION SYSTEM [J].

CARSON, S ;

COHEN, AS ;

BELENKII, A ;

RUIZMARTINEZ, MC ;

BERKA, J ;

KARGER, BL .

ANALYTICAL CHEMISTRY, 1993, 65 (22) :3219-3226

[3] 2-LABEL PEAK-HEIGHT ENCODED DNA SEQUENCING BY CAPILLARY GEL-ELECTROPHORESIS - 3 EXAMPLES [J].

CHEN, DY ;

HARKE, HR ;

DOVICHI, NJ .

NUCLEIC ACIDS RESEARCH, 1992, 20 (18) :4873-4880

[4]

GUTTMAN A, 1994, HDB CAPILLARY ELECTR, P137

[5]

*J W SCI, 1992, 2 CE J W SCI

[6]

KAPUSCINSKI J, 1990, J HISTOCHEM CYTOCHEM, V9, P1323

[7] MULTIWAVELENGTH FLUORESCENCE DETECTION FOR DNA SEQUENCING USING CAPILLARY ELECTROPHORESIS [J].

KARGER, AE ;

HARRIS, JM ;

GESTELAND, RF .

NUCLEIC ACIDS RESEARCH, 1991, 19 (18) :4955-4962

[8]

KREOITMAN M, 1989, GENE ANAL TECH, V6, P84

[9]

KUSUKAWA N, 1990, BIOTECHNIQUES, V9, P66

[10] HIGH-SPEED AND HIGH-ACCURACY DNA-SEQUENCING BY CAPILLARY GEL-ELECTROPHORESIS IN A SIMPLE, LOW-COST INSTRUMENT 2-COLOR PEAK-HEIGHT ENCODED SEQUENCING AT 40-DEGREES-C [J].

LU, H ;

ARRIAGA, E ;

CHEN, DY ;

DOVICHI, NJ .

JOURNAL OF CHROMATOGRAPHY A, 1994, 680 (02) :497-501

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