5-aminolevulinic acid induced protoporphyrin IX as a fluorescence marker for quantitative image analysis of high-grade dysplasia in Barrett's esophagus cellular models

被引：4

作者：

Yeh, Shu-Chi Allison ^{[1
]}

Sahli, Samir ^{[2
]}

Andrews, David W. ^{[3
]}

Patterson, Michael S. ^{[4
]}

Armstrong, David ^{[5
]}

Provias, John ^{[6
]}

Fang, Qiyin ^{[1
,2
]}

机构：

[1] McMaster Univ, Sch Biomed Engn, Hamilton, ON L8S 4K1, Canada

[2] McMaster Univ, Dept Engn Phys, Hamilton, ON L8S 4K1, Canada

[3] Sunnybrook Res Inst, Toronto, ON M4N 3M5, Canada

[4] McMaster Univ, Dept Med Phys & Appl Radiat Sci, Hamilton, ON L8S 4K1, Canada

[5] McMaster Univ, Dept Med, Div Gastroenterol, Hamilton, ON L8S 4K1, Canada

[6] McMaster Univ, Dept Pathol & Mol Med, Div Neuropathol, Hamilton, ON L8S 4K1, Canada

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2015年 / 20卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Barrett's esophagus; feature extraction; support vector machines; 5-aminolevulinic acid; endomicroscopy; PHOTODYNAMIC THERAPY; PHOTOSENSITIZER; HISTOLOGY; NEOPLASIA;

D O I：

10.1117/1.JBO.20.3.036010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Early detection and treatment of high-grade dysplasia (HGD) in Barrett's esophagus may reduce the risk of developing esophageal adenocarcinoma. Confocal endomicroscopy (CLE) has shown advantages over routine white-light endoscopic surveillance with biopsy for histological examination; however, CLE is compromised by insufficient contrast and by intra- and interobserver variation. An FDA-approved PDT photosensitizer was used here to reveal morphological and textural features similar to those found in histological analysis. Support vector machines were trained using the aforementioned features to obtain an automatic and robust detection of HGD. Our results showed 95% sensitivity and 87% specificity using the optimal feature combination and demonstrated the potential for extension to a three-dimensional cell model. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)

引用

页数：10

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