Identification of naringin metabolites mediated by human intestinal microbes with stable isotope-labeling method and UFLC-Q-TOF-MS/MS

被引:36
作者
Chen, Taobin [1 ,2 ]
Su, Weiwei [1 ,2 ]
Yan, Zenghao [1 ]
Wu, Hao [1 ]
Zeng, Xuan [1 ,2 ]
Peng, Wei [1 ]
Gan, Li [3 ]
Zhang, Yaohui [3 ]
Yao, Hongliang [1 ,2 ]
机构
[1] Sun Yat Sen Univ, Sch Life Sci, Guangdong Key Lab Plant Resources, Guangzhou 510275, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
[3] Artis Chem Co Ltd, Shanghai 201203, Peoples R China
关键词
Naringin; Metabolite; Human intestinal microbe; Stable isotope-labeling; UFLC-Q-TOF-MS/MS; IONIZATION MASS-SPECTROMETRY; PANAX-NOTOGINSENG SAPONINS; ACUTE LUNG INJURY; RAT URINE; CATABOLISM; PROBIOTICS; BACTERIA; COUGH; CELLS; ACID;
D O I
10.1016/j.jpba.2018.08.039
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Widely presented in medicinal plants, naringin is one of the major flavanones with various pharmaceutical bioactivities. After oral administration, naringin predominantly undergoes metabolisms mediated by liver cytochrome P450 and gut microbes, while its human microbes-mediated metabolic profiling is still largely obscure due to the endogenous interferences, which makes it extremely difficult to analyze metabolites precisely. In this study, we aim of systematically investigating the biotransformation of naringin mediated by human intestinal microbes through applying stable isotope-labeling method. [2',3',5',6'-D-4]naringin was synthesized and incubated anaerobically with human gut microbes. A total of 13 microbial metabolites were detected and identified by UFLC-Q-TOF-MS/MS, among which 5 were reported for the first time. Furthermore, the proposed metabolic pathway revealed that naringin went through extensive phase I metabolism in human intestinal microbes. Of note, diverse metabolic profiles of naringin among human participants were obtained, which could be attributed to the distinct gut microbiota compositions of individuals. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码:262 / 272
页数:11
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