Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells

被引：8

作者：

Tingey, Mark ^{[1
]}

Li, Yichen ^{[1
]}

Yang, Weidong ^{[1
]}

机构：

[1] Temple Univ, Dept Biol, Philadelphia, PA 19122 USA

来源：

STAR PROTOCOLS | 2021年 / 2卷 / 02期

基金：

美国国家卫生研究院;

关键词：

Atomic Force Microscopy (AFM); Biotechnology and bioengineering; Microbiology; Molecular Biology;

D O I：

10.1016/j.xpro.2021.100490

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-molecule fluorescence recovery after photobleaching (smFRAP) is a newly developed technique that combines single-molecule super-resolution microscopy and traditional FRAP microscopy. smFRAP enables researchers to measure the dynamics, spatial locations, and relative concentrations of proteins. Here, we describe a step-by-step protocol for smFRAP on nuclear envelope transmembrane proteins on the inner nuclear membrane and outer nuclear membrane in live cells. For complete details on the use and execution of this protocol, please refer to Mudumbi et al. (2016a, 2016b, 2020 .

引用

页数：15

共 9 条

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